LOCUS       sequence-70996-e        1128 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S
  TITLE     A modular cloning system for standardized assembly of multigene 
            constructs.
  JOURNAL   PLoS One. 2011 Feb 18;6(2):e16765. doi: 
            10.1371/journal.pone.0016765.
  PUBMED    21364738
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011-02-18"; volume: "6(2):e16765. doi"; pages: " 
            10.1371/journal.pone.0016765"
COMMENT     Sequence Label: pICH41766
FEATURES             Location/Qualifiers
     source          1..1128
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(556..573)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(556..572)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(565..587)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(781..800)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     900..922
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(960..978)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tgaccatgat tacgccaagc ttgcatgcct gcaggtcgac tctagaggat cccctttgaa
       61 gacaattacg aggatgcaca tgtgaccgag ggattgtctt cgtggaaact tgggacttca
      121 gattccaccc cttgcaagcg tggaaagcct tccgaccaag ggaagtgtcg gatgtagagg
      181 aaatggaaag tttgttctca gatggggact tgcttgattg cttcacaaga atgccagctt
      241 atgcggtaaa cgcagaggaa gatttagctg caatcaggaa aacgcccgag atggatgtcg
      301 gtcaagaagt taaagaacct gcaggagaca gaaatcaata ctcaaaccct gcagaaactt
      361 tcctcaacaa gctccacagg aaacacagta gggaggtgaa acaccaggcc gcaaagaaag
      421 ctaaacgcct agctgaaatc caggagtcaa tgagagctga aggtgatgcc gaaccaaatg
      481 aaataagcgg gggcatgggg gcaataccca gcaacgccga acttcctggg cccaaagggt
      541 accgagctcg aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
      601 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
      661 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat
      721 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
      781 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
      841 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccatctc
      901 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gtcgaaaggg
      961 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     1021 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     1081 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttca
//
