LOCUS       sequence-70997-e        1100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S
  TITLE     A modular cloning system for standardized assembly of multigene 
            constructs.
  JOURNAL   PLoS One. 2011 Feb 18;6(2):e16765. doi: 
            10.1371/journal.pone.0016765.
  PUBMED    21364738
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011-02-18"; volume: "6(2):e16765. doi"; pages: " 
            10.1371/journal.pone.0016765"
COMMENT     Sequence Label: pICH50932
FEATURES             Location/Qualifiers
     source          1..1100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(563..580)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(563..579)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(572..594)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(788..807)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     907..929
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(967..985)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tgaccatgat tacgccaagc ttgcatgcct gcaggtcgac tctagaggat cccctttggg
       61 cccaggaagt tcggcgttgc tgggtattgc ccccatgccc ccgcttattt catttggttc
      121 ggcatcacct tcagctctca ttgactcctg gatttcagct aggcgtttag ctttctttgc
      181 ggcctggtgt ttcacctccc tactgtgttt cctgtggagc ttgttgagga aagtttctgc
      241 agggtttgag tattgatttc tgtctcctgc aggttcttta acttcttgac cgacatccat
      301 ctcgggcgtt ttcctgattg cagctaaatc ttcctctgcg tttaccgcat aagctggcat
      361 tcttgtgaag caatcaagca agtccccatc tgagaacaaa ctttccattt cctctacatc
      421 cgacacttcc cttggtcgga aggctttcca cgcttgcaag gggtggaatc tgaagtccca
      481 agtttccacg aagacaatcc ctcggtcaca tgtgcatcct cggtctcagg cattgtcttc
      541 aaagggtacc gagctcgaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
      601 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
      661 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
      721 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
      781 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
      841 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
      901 ccatctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagtc
      961 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     1021 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     1081 aaatacattc aaatatgtat
//
