LOCUS       sequence-71018-e        4136 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mCherryS131C in E. coli cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Thomas CS, Glassman MJ, Olsen BD
  TITLE     Solid-state nanostructured materials from self-assembly of a 
            globular protein-polymer diblock copolymer.
  JOURNAL   ACS Nano. 2011 Jul 26;5(7):5697-707. doi: 10.1021/nn2013673. Epub 
            2011 Jun 22.
  PUBMED    21696135
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/nn2013673";
            journalName: "ACS Nano"; date: "2011-07-26- 26"; volume: "5"; issue:
            "7"; pages: "5697-707"
COMMENT     Sequence Label: mCherryS131C
FEATURES             Location/Qualifiers
     source          1..4136
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(10..24,25..30,31..47,48..54)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    30..46
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    62..78
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             97..108
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             127..144
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      883..977
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             1021..1680
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1087..1106)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1745..1831
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1878..1900)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1986..2126
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     2041..2060
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2141..2158)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2312..2900)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2392..2411)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3071..3862,3863..3931))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3694..3713
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3932..4036)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4104..4122
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
      121 ggatcgcatc accatcacca tcacggatcc atggtgtcta aaggtgaaga agataacatg
      181 gccatcatca aagaatttat gcgtttcaaa gtgcatatgg aaggtagcgt taatggccac
      241 gaatttgaaa ttgaaggcga aggtgaaggt cgtccgtatg aaggtaccca gacggcaaaa
      301 ctgaaagtga ccaaaggcgg tccgctgccg tttgcctggg atattctgtc tccgcagttc
      361 atgtatggta gtaaagcgta cgttaaacat ccggccgata tcccggatta tctgaaactg
      421 tcttttccgg aaggcttcaa atgggaacgt gtgatgaact tcgaagatgg cggtgtggtt
      481 accgttacgc aggatagctc tctgcaggat ggtgaattta tctacaaagt gaaactgcgc
      541 ggtaccaatt tcccgtgcga tggcccggtt atgcagaaga aaaccatggg ctgggaagcc
      601 agtagcgaac gtatgtaccc ggaagatggt gcactgaaag gcgaaatcaa acagcgcctg
      661 aaactgaaag atggcggtca ctatgatgca gaagtgaaaa ccacgtacaa agcgaaaaaa
      721 ccggtgcagc tgccgggtgc atacaacgtt aacatcaaac tggatatcac cagccataac
      781 gaagattata cgatcgttga acagtacgaa cgtgcagaag gtcgtcactc taccggtggt
      841 atggatgaac tgtacaaata aaagcttaat tagctgagct tggactcctg ttgatagatc
      901 cagtaatgac ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt
      961 tttttattgg tgagaatcca agctagcttg gcgagatttt caggagctaa ggaagctaaa
     1021 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa
     1081 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat
     1141 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt
     1201 cacattcttg cccgcctgat gaatgctcat ccggaatttc gtatggcaat gaaagacggt
     1261 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa
     1321 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat
     1381 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag
     1441 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg
     1501 gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc
     1561 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat
     1621 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa
     1681 tttttttaag gcagttattg gtgcccttaa acgcctgggg taatgactct ctagcttgag
     1741 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1801 gtcggtgaac gctctcctga gtaggacaaa tccgccctct agagctgcct cgcgcgtttc
     1861 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     1921 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     1981 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg
     2041 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     2101 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
     2161 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     2221 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     2281 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     2341 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     2401 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     2461 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     2521 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     2581 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     2641 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     2701 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     2761 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2821 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     2881 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     2941 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     3001 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     3061 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     3121 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     3181 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     3241 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     3301 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     3361 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     3421 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     3481 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     3541 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     3601 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     3661 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     3721 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     3781 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     3841 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     3901 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     3961 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     4021 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     4081 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt cttcac
//
