LOCUS       sequence-71019-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual expression construct expressing both dCas9VP48 and sgRNA from 
            separate promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC2-dual-dCas9VP48-sgExpression
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgctcaggcc gcctctctcg gccttggtca gattgtcgaa ctttctctgg gtaatcagct
       61 tggcgttcag cagctgccgc cagtagttct tcatcttctt cacgacctct tcggagggca
      121 cgttgtcgct cttgccccgg ttcttgtcgc ttctggtcag caccttgttg tcgatggagt
      181 cgtccttcag aaagctctga ggcacgatgg cgtccacatc gtagtcggac agccggttga
      241 tgtccagttc ctggtccacg tacatatccc gcccattctg caggtagtac aggtacagct
      301 tctcgttctg cagctgggtg ttttccacgg ggtgttcttt caggatctgg ctgcccagct
      361 ctttgatgcc ctcttcgatc cgcttcattc tctcgcggct gttcttctgt cccttctggg
      421 tggtctggtt ctctctggcc atttcgatca cgatgttctc gggcttgtgc cggcccatca
      481 ctttcacgag ctcgtccacc accttcactg tctgcaggat gcccttctta atggcggggc
      541 tgccggccag attggcaatg tgctcgtgca ggctatcgcc ctggccggac acctgggctt
      601 tctggatgtc ctctttaaag gtcaggctgt cgtcgtggat cagctgcatg aagtttctgt
      661 tggcgaagcc gtcggacttc aggaaatcca ggattgtctt gccggactgc ttgtcccgga
      721 tgccgttgat cagcttccgg ctcagcctgc cccagccggt gtatctccgc cgcttcagct
      781 gcttcatcac tttgtcgtcg aacaggtggg cataggtttt cagccgttcc tcgatcatct
      841 ctctgtcctc aaa
//
