LOCUS       sequence-71020-e         650 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual expression construct expressing both dCas9VP48 and sgRNA from 
            separate promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC2-dual-dCas9VP48-sgExpression
FEATURES             Location/Qualifiers
     source          1..650
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        111..359
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     111..131
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     282..301
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        378..453
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
       61 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt gagggcctat
      121 ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa
      181 ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat
      241 ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg
      301 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg
      361 ggtcttcgag aagacctgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt
      421 tatcaacttg aaaaagtggc accgagtcgg tgcttttttg ttttagagct agaaatagca
      481 agttaaaata aggctagtcc gtttttagcg cgtgcgccaa ttctgcagac aaatggctct
      541 agaggtaccc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      601 ccgcccattg acgtcaatag taacgccaat agggactttc cattgacgtc
//
