LOCUS       sequence-71022-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual expression construct expressing both dCas9VP48 and sgRNA from 
            separate promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC2-dual-dCas9VP48-sgExpression
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..121)
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             complement(140..160)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tttacccggg gagcatgtca aggtcaaaat cgtcaagagc gtcagcaggc agcatatcaa
       61 ggtcaaagtc gtcaagggca tcggctggga gcatgtctaa gtcaaaatcg tcaagggcgt
      121 cggccggccc gctggcctcc acctttctct tcttcttggg gctgtcgcct cccagctgag
      181 acaggtcgat ccgtgtctcg tacaggccgg tgatgctctg gtggatcagg gtggcgtcca
      241 gcacctcttt ggtgctggtg tacctcttcc ggtcgatggt ggtgtcaaag tacttgaagg
      301 cggcaggggc tcccagattg gtcagggtaa acaggtggat gatattctcg gcctgctctc
      361 tgatgggctt atcccggtgc ttgttgtagg cggacagcac tttgtccaga ttagcgtcgg
      421 ccaggatcac tctcttggag aactcgctga tctgctcgat gatctcgtcc aggtagtgct
      481 tgtgctgttc cacaaacagc tgtttctgct cattatcctc gggggagccc ttcagcttct
      541 catagtggct ggccaggtac aggaagttca catatttgga gggcagggcc agttcgtttc
      601 ccttctgcag ttcgccggca gaggccagca ttctcttccg gccgttttcc agctcgaaca
      661 gggagtactt aggcagcttg atgatcaggt cctttttcac ttctttgtag cccttggctt
      721 ccagaaagtc gatgggattc ttctcgaagc tgcttctttc catgatggtg atccccagca
      781 gctctttcac actcttcagt ttcttggact tgcccttttc cactttggcc accaccagca
      841 cagaata
//
