LOCUS       sequence-71023-e         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note:
            This is being tested..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC94-pmax-dCas9VP160-2A-puro
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(59..442)
                     /codon_start=1
                     /product="10 tandem repeats of the minimal activation 
                     domain of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP160
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGS
                     DALDDFDLDMLGSDALDDFDLDML"
     CDS             complement(476..496)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cgtcaccgca tgttagaagg cttcctctgc cctctccgct gccagatcta tcgatataca
       61 acatatccaa atcgaagtca tcgagcgcat cggagcccag catgtccagg tcaaagtcgt
      121 ccagggcatc actccccagc atatccaagt caaaatcatc caaggcatcg gaccccagca
      181 tgtccagatc gaaatcgtcc agggcgtcgc tgccgagcat gtccaggtcg aaatcatcaa
      241 gggcatcgct tcccaacata tcaaggtcga aatcatccag tgcgtctgac cctaacatat
      301 cgagatcgaa atcgtccaga gcatcggagc cgagcatgtc cagatcaaag tcatccaatg
      361 cgtcgcttcc caacatatcc aggtcaaagt catcgagggc atcagaaccc agcatgtcga
      421 gatcgaaatc gtccagcgcg tcggcgcgcc cggatccggc cggcccgctg gcctccacct
      481 ttctcttctt cttggggctg tcgcctccca gctgagacag gtcgatccgt gtctcgtaca
      541 ggccggtgat gctctggtgg atcagggtgg cgtccagcac ctctttggtg ctggtgtacc
      601 tcttccggtc gatggtggtg tcaaagtact tgaaggcggc aggggctccc agattggtca
      661 gggtaaacag gtggatgata ttctcggcct gctctctgat gggcttatcc cggtgcttgt
      721 tgtaggcgga cagcactttg tccagattag cgtcggccag gatcactctc ttggagaact
      781 cgctgatctg ctcgatgatc tcgtccaggt agtgcttgtg ctgttccaca aacagctgtt
      841 tctgctcatt atcctcgggg gagcccttca gcttctcata gtggctggcc aggtacagga
      901 agttcacata tttgg
//
