LOCUS       sequence-71025-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note:
            This is being tested..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC94-pmax-dCas9VP160-2A-puro
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..72
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             76..96
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agtttgtaca aaaaagcagg ctccgaattc accggtgcca ccatgtaccc atacgatgtt
       61 ccagattacg cttcgccgaa gaaaaagcgc aaggtcgaag cgtccgacaa gaagtacagc
      121 atcggcctgg ccatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag
      181 gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac
      241 ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga
      301 accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc
      361 agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg
      421 gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg
      481 gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc
      541 gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc
      601 cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc
      661 cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg
      721 gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc
      781 gcccagctgc ccggcgagaa gaagaatggc ctgttcggca acctgattgc cctgagcctg
      841 ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg
      901 agcaaggaca cctacgacga cg
//
