LOCUS       sequence-71026-e         742 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual expression construct expressing both dCas9VP48 and sgTetO from 
            separate promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC5-dual-dCas9VP48-sgTetO
FEATURES             Location/Qualifiers
     source          1..742
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        112..360
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     112..132
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     283..302
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        381..456
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
       61 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg tgagggccta
      121 tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga gataattgga
      181 attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa
      241 tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc
      301 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc
      361 gcttttctct atcactgata gttttagagc tagaaatagc aagttaaaat aaggctagtc
      421 cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgttttaga gctagaaata
      481 gcaagttaaa ataaggctag tccgttttta gcgcgtgcgc caattctgca gacaaatggc
      541 tctagaggta cccgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      601 cccccgccca ttgacgtcaa tagtaacgcc aatagggact ttccattgac gtcaatgggt
      661 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      721 gccccctatt gacgtcaatg ac
//
