LOCUS       sequence-71027-e         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual expression construct expressing both dCas9VP48 and sgTetO from 
            separate promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC5-dual-dCas9VP48-sgTetO
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gccgcctctc tcggccttgg tcagattgtc gaactttctc tgggtaatca gcttggcgtt
       61 cagcagctgc cgccagtagt tcttcatctt cttcacgacc tcttcggagg gcacgttgtc
      121 gctcttgccc cggttcttgt cgcttctggt cagcaccttg ttgtcgatgg agtcgtcctt
      181 cagaaagctc tgaggcacga tggcgtccac atcgtagtcg gacagccggt tgatgtccag
      241 ttcctggtcc acgtacatat cccgcccatt ctgcaggtag tacaggtaca gcttctcgtt
      301 ctgcagctgg gtgttttcca cggggtgttc tttcaggatc tggctgccca gctctttgat
      361 gccctcttcg atccgcttca ttctctcgcg gctgttcttc tgtcccttct gggtggtctg
      421 gttctctctg gccatttcga tcacgatgtt ctcgggcttg tgccggccca tcactttcac
      481 gagctcgtcc accaccttca ctgtctgcag gatgcccttc ttaatggcgg ggctgccggc
      541 cagattggca atgtgctcgt gcaggctatc gccctggccg gacacctggg ctttctggat
      601 gtcctcttta aaggtcaggc tgtcgtcgtg gatcagctgc atgaagtttc tgttggcgaa
      661 gccgtcggac ttcaggaaat ccaggattgt cttgccggac tgcttgtccc ggatgccgtt
      721 gatcagcttc cggctcagcc tgccccagcc ggtgtatctc cgccgcttca gctgcttcat
      781 cac
//
