LOCUS       sequence-71029-e        1019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual expression construct expressing both dCas9VP48 and sgTetO from 
            separate promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC5-dual-dCas9VP48-sgTetO
FEATURES             Location/Qualifiers
     source          1..1019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(10..120)
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             complement(139..159)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttacccgggg agcatgtcaa ggtcaaaatc gtcaagagcg tcagcaggca gcatatcaag
       61 gtcaaagtcg tcaagggcat cggctgggag catgtctaag tcaaaatcgt caagggcgtc
      121 ggccggcccg ctggcctcca cctttctctt cttcttgggg ctgtcgcctc ccagctgaga
      181 caggtcgatc cgtgtctcgt acaggccggt gatgctctgg tggatcaggg tggcgtccag
      241 cacctctttg gtgctggtgt acctcttccg gtcgatggtg gtgtcaaagt acttgaaggc
      301 ggcaggggct cccagattgg tcagggtaaa caggtggatg atattctcgg cctgctctct
      361 gatgggctta tcccggtgct tgttgtaggc ggacagcact ttgtccagat tagcgtcggc
      421 caggatcact ctcttggaga actcgctgat ctgctcgatg atctcgtcca ggtagtgctt
      481 gtgctgttcc acaaacagct gtttctgctc attatcctcg ggggagccct tcagcttctc
      541 atagtggctg gccaggtaca ggaagttcac atatttggag ggcagggcca gttcgtttcc
      601 cttctgcagt tcgccggcag aggccagcat tctcttccgg ccgttttcca gctcgaacag
      661 ggagtactta ggcagcttga tgatcaggtc ctttttcact tctttgtagc ccttggcttc
      721 cagaaagtcg atgggattct tctcgaagct gcttctttcc atgatggtga tccccagcag
      781 ctctttcaca ctcttcagtt tcttggactt gcccttttcc actttggcca ccaccagcac
      841 agaataggcc acggtggggc tgtcgaagcc gccgtacttc ttagggtccc agtccttctt
      901 tctggcgatc agcttatcgc tgttcctctt gggcaggata gactctttgc tgaagccgcc
      961 tgtctgcacc tcggtctttt tcacgatatt cacttggggc atgctcagca ctttccgca
//
