LOCUS       sequence-71032-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual expression construct expressing both dCas9VP96 and sgRNA from 
            separate promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC153-dual-dCas9VP96-sgExpression
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcgctcaggc cgcctctctc ggccttggtc agattgtcga actttctctg ggtaatcagc
       61 ttggcgttca gcagctgccg ccagtagttc ttcatcttct tcacgacctc ttcggagggc
      121 acgttgtcgc tcttgccccg gttcttgtcg cttctggtca gcaccttgtt gtcgatggag
      181 tcgtccttca gaaagctctg aggcacgatg gcgtccacat cgtagtcgga cagccggttg
      241 atgtccagtt cctggtccac gtacatatcc cgcccattct gcaggtagta caggtacagc
      301 ttctcgttct gcagctgggt gttttccacg gggtgttctt tcaggatctg gctgcccagc
      361 tctttgatgc cctcttcgat ccgcttcatt ctctcgcggc tgttcttctg tcccttctgg
      421 gtggtctggt tctctctggc catttcgatc acgatgttct cgggcttgtg ccggcccatc
      481 actttcacga gctcgtccac caccttcact gtctgcagga tgcccttctt aatggcgggg
      541 ctgccggcca gattggcaat gtgctcgtgc aggctatcgc cctggccgga cacctgggct
      601 ttctggatgt cctctttaaa ggtcaggctg tcgtcgtgga tcagctgcat gaagtttctg
      661 ttggcgaagc cgtcggactt caggaaatcc aggattgtct tgccggactg cttgtcccgg
      721 atgccgttga tcagcttccg gctcagcctg ccccagccgg tgtatctccg ccgcttcagc
      781 tgcttcatca ctttgtcgtc gaacaggtgg gcataggttt tcagccgttc ctcgatcatc
      841 tctctgtcct caaacagtgt
//
