LOCUS       sequence-71033-e         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual expression construct expressing both dCas9VP96 and sgRNA from 
            separate promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC153-dual-dCas9VP96-sgExpression
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..247)
                     /codon_start=1
                     /product="6 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP96
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDMLGSDALDDFDLDMLGSDALDDFDLDML"
     CDS             complement(281..301)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttattaatca atcgatatac aacatatcca aatcgaaatc atcaagggca tcgcttccca
       61 acatatcaag gtcgaaatca tccagtgcgt ctgaccctaa catatcgaga tcgaaatcgt
      121 ccagagcatc ggagccgagc atgtccagat caaagtcatc caatgcgtcg cttcccaaca
      181 tatccaggtc aaagtcatcg agggcatcag aacccagcat gtcgagatcg aaatcgtcca
      241 gcgcgtcggc gcgcccggat ccggccggcc cgctggcctc cacctttctc ttcttcttgg
      301 ggctgtcgcc tcccagctga gacaggtcga tccgtgtctc gtacaggccg gtgatgctct
      361 ggtggatcag ggtggcgtcc agcacctctt tggtgctggt gtacctcttc cggtcgatgg
      421 tggtgtcaaa gtacttgaag gcggcagggg ctcccagatt ggtcagggta aacaggtgga
      481 tgatattctc ggcctgctct ctgatgggct tatcccggtg cttgttgtag gcggacagca
      541 ctttgtccag attagcgtcg gccaggatca ctctcttgga gaactcgctg atctgctcga
      601 tgatctcgtc caggtagtgc ttgtgctgtt ccacaaacag ctgtttctgc tcattatcct
      661 cgggggagcc cttcagcttc tcatagtggc tggccaggta caggaagttc acatatttgg
      721 agggcagggc cagttcgttt cccttctgca gttcgccggc aga
//
