LOCUS       sequence-71040-e        5198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HA-I-SceI expression plasmid, episomal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Gupta R, Barkan D, Redelman-Sidi G, Shuman S, Glickman MS
  TITLE     Mycobacteria exploit three genetically distinct DNA double-strand 
            break repair pathways.
  JOURNAL   Mol Microbiol. 2011 Jan;79(2):316-30. doi: 
            10.1111/j.1365-2958.2010.07463.x. Epub 2010 Nov 24.
  PUBMED    21219454
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1365-2958.2010.07463.x"; journalName: "Mol Microbiol"; 
            date: "2011-01"; volume: "79"; issue: "2"; pages: "316-30"
COMMENT     Sequence Label: pRGM1
FEATURES             Location/Qualifiers
     source          1..5198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(17..22,23..40,41..47)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    53..72
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     CDS             109..135
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      1066..1112
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      2498..3086
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2987..3006
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gatccagacc ccaggcttga cactttatgc ttccggctcg tataatgtgt ggaattgtga
       61 gcgctcacaa ttcgcatgca gaaaggaggc catatgggac gatttatgta cccatacgat
      121 gttcctgact atgcgggtat gaaaaacatc aaaaaaaacc aggtaatgaa cctgggtccg
      181 aactctaaac tgctgaaaga atacaaatcc cagctgatcg aactgaacat cgaacagttc
      241 gaagcaggta tcggtctgat cctgggtgat gcttacatcc gttctcgtga tgaaggtaaa
      301 acctactgta tgcagttcga gtggaaaaac aaagcataca tggaccacgt atgtctgctg
      361 tacgatcagt gggtactgtc cccgccgcac aaaaaagaac gtgttaacca cctgggtaac
      421 ctggtaatca cctggggcgc ccagactttc aaacaccaag ctttcaacaa actggctaac
      481 ctgttcatcg ttaacaacaa aaaaaccatc ccgaacaacc tggttgaaaa ctacctgacc
      541 ccgatgtctc tggcatactg gttcatggat gatggtggta aatgggatta caacaaaaac
      601 tctaccaaca aatcgatcgt actgaacacc cagtctttca ctttcgaaga agtagaatac
      661 ctggttaagg gtctgcgtaa caaattccaa ctgaactgtt acgtaaaaat caacaaaaac
      721 aaaccgatca tctacatcga ttctatgtct tacctgatct tctacaacct gatcaaaccg
      781 tacctgatcc cgcagatgat gtacaaactg ccgaacacta tctcctccga aactttcctg
      841 aaataataag tcgatcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt
      901 ggctggtgtg gaaacgcggc cgcacgcgtt gcgctcggtc gttcggctgc ggcgagcggt
      961 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     1021 gaacatggat gaattcgaag cttatcgatg tcgactgcca ggcatcaaat aaaacgaaag
     1081 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtccggcca tcatggccgc
     1141 ggtgatcagc tagaggggcg tcaggcgccg ggggcggtgt ccggcggccc ccagaggaac
     1201 tgcgccagtt cctccggatc ggtgaagccg gagagatcca gcggggtctc ctcgaacacc
     1261 tcgaagtcgt gcaggaaggt gaaggcgagc agttcgcggg cgaagtcctc ggtccgcttc
     1321 cactgcgccc cgtcgagcag cgcggccagg atctcgcggt cgccccggaa ggcgttgaga
     1381 tgcagttgca ccaggctgta gcgggagtct cccgcataga cgtcggtgaa gtcgacgatc
     1441 ccggtgacct cggtcgcggc caggtccacg aagatgttgg tcccgtgcag gtcgccgtgg
     1501 acgaaccggg gttcgcggcc ggccagcagc gtgtccacgt ccggcagcca gtcctccagg
     1561 cggtccagca gccggggcga gaggtagccc cacccgcggt ggtcctcgac ggtcgccgcg
     1621 cggcgttccc gcagcagttc cgggaagacc tcggaatggg gggtgagcac ggtgttcccg
     1681 gtcagcggca ccctgtgcag ccggccgagc acccggccga gttcgcgggc cagggcgagc
     1741 agcgcgttcc ggtcggtcgt gccgtccatc gcggaccgcc aggtggtgcc ggtcatccgg
     1801 ctcatcacca ggtagggcca cggccaggct ccggtgccgg gccgcagctc gccgcggccg
     1861 aggaggcggg gcaccggcac cggggcgtcc gccaggaccg cgtacgcctc cgactccgac
     1921 gcgaggctct ccggaccgca ccagtgctcg ccgaacagct tgatcaccgg gtcgggctcg
     1981 ccgaccagta cggggttggt gctctcgccg ggcacccgca gcaccggcgg caccggcagc
     2041 ccgagctcct ccagggctcg gcgggccagc ggctcccaga attcctggtc gttccgcagg
     2101 ctcgcgtagg aatcatccga atcaatacgg tcgagaagta acagggattc ttgtgtcaca
     2161 gcggacctct attcacaggg tacgggccgg cttaattccg cacggccggt cgcgacacgg
     2221 cctgtccgca ccgcggtcag gcgttgacga tgacgggctg gtcggccacg tcggggagtc
     2281 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     2341 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     2401 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgctc tagttccact
     2461 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2521 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2581 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2641 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2701 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2761 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2821 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2881 agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2941 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3001 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3061 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     3121 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     3181 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     3241 acgcgtgcgg ccgcacggcg tgagcccacc agctccgtaa gttcgggtgc tgtgtggctc
     3301 gtacccgcgc attcaggcgg cagggggtct aacgggtcta aggcggcgtg tacggccgcc
     3361 acagcggctc ttagcggccc ggaaacgtcc tcgaaacgac gcatgtgttc ctcctggttg
     3421 gtacaggtgg ttgggggtgc tcggctgtcg ctggtgtttc atcatcaggg ctcgacggga
     3481 gagcggggga gtgtgcagtt gtggggtggc ccctcagcga aatatctgac ttggagctcg
     3541 tgtcggacca tacaccggtg attaatcgtg gtttattatc aagcgtgagc cacgtcgccg
     3601 acgaatttga gcagctctgg ctgccgtact ggtccctggc aagcgacgat ctgctcgagg
     3661 ggatctaccg ccaaagccgc gcgtcggccc taggccgccg gtacatcgag gcgaacccaa
     3721 cagcgctggc aaacctgctg gtcgtggacg tagaccatcc agacgcagcg ctccgagcgc
     3781 tcagcgcccg ggggtcccat ccgctgccca acgcgatcgt gggcaatcgc gccaacggcc
     3841 acgcacacgc agtgtgggca ctcaacgccc ctgttccacg caccgaatac gcgcggcgta
     3901 agccgctcgc atacatggcg gcgtgcgccg aaggccttcg gcgcgccgtc gatggcgacc
     3961 gcagttactc aggcctcatg accaaaaacc ccggccacat cgcctgggaa acggaatggc
     4021 tccactccga tctctacaca ctcagccaca tcgaggccga gctcggcgcg aacatgccac
     4081 cgccgcgctg gcgtcagcag accacgtaca aagcggctcc gacgccgcta gggcggaatt
     4141 gcgcactgtt cgattccgtc aggttgtggg cctatcttcc cgccctcatg cggatctacc
     4201 tgccgacccg gaacgtggac ggactcggcc gcgcgatcta tgccgagtgc cacgcgcgaa
     4261 acgccgaatt tccgtgcaac gacgtgtgtc ccggaccgct accggacagc gaggtccgcg
     4321 ccatcgccaa cagcatttgg cgttggatca caaccaagtc gcgcatttgg gcggacggga
     4381 tcgtggtcta cgaggccaca ctcagtgcgc gccatgcggc catctcgcgg aagggcgcag
     4441 cagcgcgcac ggcggcgagc acagttgcgc ggcgcgcaaa gtccgcgtca gccatgcatg
     4501 gaggcattgc tatgagcgac ggctacagcg acggctacag cgacggctac aactggcagc
     4561 cgactgtccg caaaaagcgg cgcgtgaccg ccgccgaagg cgctcgaatc accggactat
     4621 ccgaacgcca cgtcgtccgg ctcgtggcgc aggaacgcag cgagtggttc gccgagcagg
     4681 ctgcacgccg cgaacgcatc cgcgcctatc acgacgacga gggccactct tggccgcaaa
     4741 cggccaaaca tttcgggctg catctggaca ccgttaagcg actcggctat cgggcgagga
     4801 aagagcgtgc ggcagaacag gaagcggctc aaaaggccca caacgaagcc gacaatccac
     4861 cgctgttcta acgcaattgg ggagcgggtg tcgcgggggt tccgtggggg gttccgttgc
     4921 aacgggtcgg acaggtaaaa gtcctggtag acgctagttt tctggtttgg gccatgcctg
     4981 tctcgttgcg tgtttcgttg cgtccgtttt gaataccagc cagacgagac ggggttctac
     5041 gaatcttggt cgataccaag ccatttccgc tgaatatcgt ggagctcacc gccagaatcg
     5101 gtggttgtgg tgatgtacgt ggcgaactcc gttgtagtgc ttgtggtggc atccgtggcg
     5161 cggccgcggt accagatctt taaatctaga tatccatg
//
