LOCUS       sequence-71054-e         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9VP160 on pmax expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC93-pmax-dCas9VP160
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    86..110
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(138..521)
                     /codon_start=1
                     /product="10 tandem repeats of the minimal activation 
                     domain of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP160
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGS
                     DALDDFDLDMLGSDALDDFDLDML"
     CDS             complement(555..575)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gagaccgagg agagggttag ggataggctt accttcgaac cgcgggccct ctagactcga
       61 gcggccgcca ctgtgctgga tatcaaccac tttgtacaag aaagctgggt cgaattctta
      121 attaatcaat cgatatacaa catatccaaa tcgaagtcat cgagcgcatc ggagcccagc
      181 atgtccaggt caaagtcgtc cagggcatca ctccccagca tatccaagtc aaaatcatcc
      241 aaggcatcgg accccagcat gtccagatcg aaatcgtcca gggcgtcgct gccgagcatg
      301 tccaggtcga aatcatcaag ggcatcgctt cccaacatat caaggtcgaa atcatccagt
      361 gcgtctgacc ctaacatatc gagatcgaaa tcgtccagag catcggagcc gagcatgtcc
      421 agatcaaagt catccaatgc gtcgcttccc aacatatcca ggtcaaagtc atcgagggca
      481 tcagaaccca gcatgtcgag atcgaaatcg tccagcgcgt cggcgcgccc ggatccggcc
      541 ggcccgctgg cctccacctt tctcttcttc ttggggctgt cgcctcccag ctgagacagg
      601 tcgatccgtg tctcgtacag gccggtgatg ctctggtgga tcagggtggc gtccagcacc
      661 tctttggtgc tggtgtacct cttccggtcg atggtggtgt caaagtactt gaaggcggca
      721 ggggctccca gattggtcag ggtaaacagg tggatgatat tctcggcctg ctctctgatg
      781 ggcttatccc ggtgctt
//
