LOCUS       sequence-71058-e         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Destination vector derived from Clontech's pmax (CAGGS) 
            expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC90-pmax-DEST
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          95..227
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     protein_bind    242..366
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(391..396,397..414,415..421)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(541..560)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ggtcgtttag tgaaccgtca gatcactagt agctttattg cggtagttta tcacagttaa
       61 attgctaacg cagtcagtgc tcgactgatc acaggtaagt atcaaggtta caagacaggt
      121 ttaaggaggc caatagaaac tgggcttgtc gagacagaga agattcttgc gtttctgata
      181 ggcacctatt ggtcttactg acatccactt tgcctttctc tccacaggga aaaaacaatt
      241 gacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt
      301 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc
      361 actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat
      421 gtgtggattt tgagttagga tccgtcgaga ttttcaggag ctaaggaagc taaaatggag
      481 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt
      541 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg
      601 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt
      661 cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg
      721 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt
      781 tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa
      841 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg
      901 tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat
      961 atggacaact t
//
