LOCUS       sequence-71059-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Destination vector derived from Clontech's pmax (CAGGS) 
            expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC90-pmax-DEST
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(111..235)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             288..329
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(355..374)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    384..465
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     409..428
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(743..762)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
       61 gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact
      121 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
      181 tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggttgata
      241 tccagcacag tggcggccgc tcgagtctag agggcccgcg gttcgaaggt aagcctatcc
      301 ctaaccctct cctcggtctc gattctacgc gtaccggtta gcaattgttt tttcgatgag
      361 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
      421 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      481 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      541 ctctacaaat gtggtactta agagggggag accaaagggc gagacgttaa ggcctcacgt
      601 gacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      661 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      721 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      781 cgctctcctg ttccgaccct
//
