LOCUS       sequence-71062-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not 
            for expression. It has to be transferred to a gateway destination 
            vector for expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC149-pCR8-dCas9VP160
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    74..98
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             122..148
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             152..172
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg atgagcaatg
       61 cttttttata atgccaactt tgtacaaaaa agcaggctcc gaattcaccg gtgccaccat
      121 gtacccatac gatgttccag attacgcttc gccgaagaaa aagcgcaagg tcgaagcgtc
      181 cgacaagaag tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat
      241 caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca
      301 cagcatcaag aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc
      361 cacccggctg aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta
      421 tctgcaagag atcttcagca acgagatggc caaggtggac gacagcttct tccacagact
      481 ggaagagtcc ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa
      541 catcgtggac gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa
      601 actggtggac agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat
      661 gatcaagttc cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt
      721 ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat
      781 caacgccagc ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg
      841 gctggaaaat ctgatcgccc agctgcccgg cgagaa
//
