LOCUS       sequence-71063-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9VP96 on Gateway donor vector pCR8/GW/TOPO. Note: This is not 
            for expression. It has to be transferred to a gateway destination 
            vector for expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC148-pCR8-dCas9VP96
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    12..111
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(139..366)
                     /codon_start=1
                     /product="6 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP96
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDMLGSDALDDFDLDMLGSDALDDFDLDML"
     CDS             complement(400..420)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cagctggatg gcaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa
       61 ttgatgagca attatttttt ataatgccaa ctttgtacaa gaaagctggg tcgaattctt
      121 aattaatcaa tcgatataca acatatccaa atcgaaatca tcaagggcat cgcttcccaa
      181 catatcaagg tcgaaatcat ccagtgcgtc tgaccctaac atatcgagat cgaaatcgtc
      241 cagagcatcg gagccgagca tgtccagatc aaagtcatcc aatgcgtcgc ttcccaacat
      301 atccaggtca aagtcatcga gggcatcaga acccagcatg tcgagatcga aatcgtccag
      361 cgcgtcggcg cgcccggatc cggccggccc gctggcctcc acctttctct tcttcttggg
      421 gctgtcgcct cccagctgag acaggtcgat ccgtgtctcg tacaggccgg tgatgctctg
      481 gtggatcagg gtggcgtcca gcacctcttt ggtgctggtg tacctcttcc ggtcgatggt
      541 ggtgtcaaag tacttgaagg cggcaggggc tcccagattg gtcagggtaa acaggtggat
      601 gatattctcg gcctgctctc tgatgggctt atcccggtgc ttgttgtagg cggacagcac
      661 tttgtccaga ttagcgtcgg ccaggatcac tctcttggag aactcgctga tctgctcgat
      721 gatctcgtcc aggtagtgct tgtgctgttc cacaaacagc tgtttctgct cattatcctc
      781 gggggagccc ttcagcttct catagtggct ggccaggtac aggaagttca catatttgga
      841 gggcagggcc agttcgtttc ccttctgcag ttcgccggca gaggccagca ttctcttccg
      901 gccgttttcc agctcgaaca ggg
//
