LOCUS       sequence-71065-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9VP96 on Gateway donor vector pCR8/GW/TOPO. Note: This is not 
            for expression. It has to be transferred to a gateway destination 
            vector for expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC148-pCR8-dCas9VP96
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             118..144
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             148..168
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
       61 tttataatgc caactttgta caaaaaagca ggctccgaat tcaccggtgc caccatgtac
      121 ccatacgatg ttccagatta cgcttcgccg aagaaaaagc gcaaggtcga agcgtccgac
      181 aagaagtaca gcatcggcct ggccatcggc accaactctg tgggctgggc cgtgatcacc
      241 gacgagtaca aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc
      301 atcaagaaga acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc
      361 cggctgaaga gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg
      421 caagagatct tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa
      481 gagtccttcc tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc
      541 gtggacgagg tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg
      601 gtggacagca ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc
      661 aagttccggg gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac
      721 aagctgttca tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac
      781 gccagcggcg tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg
      841 gaaaatctga tcgcccagct gcccggcgag aagaagaatg gcctgttcgg caacctgatt
      901 gccctgagcc tgggcctgac ccccaacttc aagagcaact tcg
//
