LOCUS       sequence-71066-e         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9VP64 on Gateway donor vector pCR8/GW/TOPO. Note: This is not 
            for expression. It has to be transferred to a gateway destination 
            vector for expression .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC147-pCR8-dCas9VP64
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..110
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(138..287)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(321..341)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agctggatgg caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat
       61 tgatgagcaa ttatttttta taatgccaac tttgtacaag aaagctgggt cgaattctta
      121 attaatcaat cgatatataa catatcgaga tcgaaatcgt ccagagcatc ggagccgagc
      181 atgtccagat caaagtcatc caatgcgtcg cttcccaaca tatccaggtc aaagtcatcg
      241 agggcatcag aacccagcat gtcgagatcg aaatcgtcca gcgcgtcggc gcgcccggat
      301 ccggccggcc cgctggcctc cacctttctc ttcttcttgg ggctgtcgcc tcccagctga
      361 gacaggtcga tccgtgtctc gtacaggccg gtgatgctct ggtggatcag ggtggcgtcc
      421 agcacctctt tggtgctggt gtacctcttc cggtcgatgg tggtgtcaaa gtacttgaag
      481 gcggcagggg ctcccagatt ggtcagggta aacaggtgga tgatattctc ggcctgctct
      541 ctgatgggct tatcccggtg cttgttgtag gcggacagca ctttgtccag attagcgtcg
      601 gccaggatca ctctcttgga gaactcgctg atctgctcga tgatctcgtc caggtagtgc
      661 ttgtgctgtt ccacaaacag ctgtttctgc tcattatcct cgggggagcc cttcagcttc
      721 tcatagtggc tggccaggta caggaagttc acatatttgg agggcagggc cagttcgttt
      781 cccttctgca gttcgccggc a
//
