LOCUS       sequence-71069-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for 
            expression. It has to be transferred to a gateway destination vector
            for expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC84-pCR8-dCas9
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    12..111
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(131..151)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cagctggatg gcaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa
       61 ttgatgagca attatttttt ataatgccaa ctttgtacaa gaaagctggg tcgaattctt
      121 agctggcctc cacctttctc ttcttcttgg ggctgtcgcc tcccagctga gacaggtcga
      181 tccgtgtctc gtacaggccg gtgatgctct ggtggatcag ggtggcgtcc agcacctctt
      241 tggtgctggt gtacctcttc cggtcgatgg tggtgtcaaa gtacttgaag gcggcagggg
      301 ctcccagatt ggtcagggta aacaggtgga tgatattctc ggcctgctct ctgatgggct
      361 tatcccggtg cttgttgtag gcggacagca ctttgtccag attagcgtcg gccaggatca
      421 ctctcttgga gaactcgctg atctgctcga tgatctcgtc caggtagtgc ttgtgctgtt
      481 ccacaaacag ctgtttctgc tcattatcct cgggggagcc cttcagcttc tcatagtggc
      541 tggccaggta caggaagttc acatatttgg agggcagggc cagttcgttt cccttctgca
      601 gttcgccggc agaggccagc attctcttcc ggccgttttc cagctcgaac agggagtact
      661 taggcagctt gatgatcagg tcctttttca cttctttgta gcccttggct tccagaaagt
      721 cgatgggatt cttctcgaag ctgcttcttt ccatgatggt gatccccagc agctctttca
      781 cactcttcag tttcttggac ttgccctttt ccactttggc caccaccagc acagaatagg
      841 ccacggtggg gctgtcgaag ccgccgtact t
//
