LOCUS       sequence-71070-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for 
            expression. It has to be transferred to a gateway destination vector
            for expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC84-pCR8-dCas9
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttcgctcagg ccgcctctct cggccttggt cagattgtcg aactttctct gggtaatcag
       61 cttggcgttc agcagctgcc gccagtagtt cttcatcttc ttcacgacct cttcggaggg
      121 cacgttgtcg ctcttgcccc ggttcttgtc gcttctggtc agcaccttgt tgtcgatgga
      181 gtcgtccttc agaaagctct gaggcacgat ggcgtccaca tcgtagtcgg acagccggtt
      241 gatgtccagt tcctggtcca cgtacatatc ccgcccattc tgcaggtagt acaggtacag
      301 cttctcgttc tgcagctggg tgttttccac ggggtgttct ttcaggatct ggctgcccag
      361 ctctttgatg ccctcttcga tccgcttcat tctctcgcgg ctgttcttct gtcccttctg
      421 ggtggtctgg ttctctctgg ccatttcgat cacgatgttc tcgggcttgt gccggcccat
      481 cactttcacg agctcgtcca ccaccttcac tgtctgcagg atgcccttct taatggcggg
      541 gctgccggcc agattggcaa tgtgctcgtg caggctatcg ccctggccgg acacctgggc
      601 tttctggatg tcctctttaa aggtcaggct gtcgtcgtgg atcagctgca tgaagtttct
      661 gttggcgaag ccgtcggact tcaggaaatc caggattgtc ttgccggact gcttgtcccg
      721 gatgccgttg atcagcttcc ggctcagcct gccccagccg gtgtatctcc gccgcttcag
      781 ctgcttcatc actttgtcgt cgaacaggtg ggcataggtt ttcagccgtt cctcgatcat
      841 ctctctgtcc tcaaa
//
