LOCUS       sequence-71071-e         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for 
            expression. It has to be transferred to a gateway destination vector
            for expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC84-pCR8-dCas9
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    73..97
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             127..153
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             157..177
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga tgagcaatgc
       61 ttttttataa tgccaacttt gtacaaaaaa gcaggctccg aattcgccct taccggtgcc
      121 accatgtacc catacgatgt tccagattac gcttcgccga agaaaaagcg caaggtcgaa
      181 gcgtccgaca agaagtacag catcggcctg gccatcggca ccaactctgt gggctgggcc
      241 gtgatcaccg acgagtacaa ggtgcccagc aagaaattca aggtgctggg caacaccgac
      301 cggcacagca tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc
      361 gaggccaccc ggctgaagag aaccgccaga agaagataca ccagacggaa gaaccggatc
      421 tgctatctgc aagagatctt cagcaacgag atggccaagg tggacgacag cttcttccac
      481 agactggaag agtccttcct ggtggaagag gataagaagc acgagcggca ccccatcttc
      541 ggcaacatcg tggacgaggt ggcctaccac gagaagtacc ccaccatcta ccacctgaga
      601 aagaaactgg tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc
      661 cacatgatca agttccgggg ccacttcctg atcgagggcg acctgaaccc cgacaacagc
      721 gacgtggaca agctgttcat ccagctggtg cagacctaca accagctgtt cgaggaaaac
      781 cccatcaacg ccagcggcgt ggacgccaag gccatcctgt ctgccagact gagcaagagc
      841 agacggctgg aaaatctgat cgcccagctg cccggcgaga agaagaatgg cctgttcggc
      901 aacctgattg ccctgagcct gggcctgacc cccaacttca agagcaactt cgacctggcc
      961 gaggatgcca
//
