LOCUS       sequence-71076-e        1047 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An AAV vector that expresses iRFP under the c-fos promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Richie CT, Whitaker LR, Whitaker KW, Necarsulmer J, Baldwin HA, 
            Zhang Y, Fortuno L, Hinkle JJ, Koivula P, Henderson MJ, Sun W, Wang 
            K, Smith JC, Pickel J, Ji N, Hope BT, Harvey BK
  TITLE     Near-infrared fluorescent protein iRFP713 as a reporter protein for 
            optogenetic vectors, a transgenic Cre-reporter rat, and other 
            neuronal studies.
  JOURNAL   J Neurosci Methods. 2017 Jun 1;284:1-14. doi: 
            10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2.
  PUBMED    28380331
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci 
            Methods."; date: "2017-06-1"; volume: "284:1-14. doi"; pages: " 
            10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2"
COMMENT     Sequence Label: pOTTC475 - pAAV c-fos iRFP
FEATURES             Location/Qualifiers
     source          1..1047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(77..1024)
                     /codon_start=1
                     /product="phytochrome-based near-infrared fluorescent 
                     protein, originally termed iRFP (Filonov et al., 2011)"
                     /label=iRFP713
                     /note="name was changed to iRFP713 (Shcherbakova and 
                     Verkhusha, 2013)"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
ORIGIN
        1 cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt atcgataagc ttgatatcga
       61 attcttactt gtacaactct tccatcacgc cgatctgcca ggccagaacc tgggcgacta
      121 gctcgcaggc ttggcggcca tcgagatcga cgtagtacgg cgttcggtga tggcaaacga
      181 tcaatcccca cagtcgctcg ccgcgcaaaa tcgagatcga catcgtgccg tgcatgccta
      241 tgttgcgcat gaattccaga tggacgggcg agacgctgcg caggatggcg aagctaagat
      301 caatcggccg cccggtgacc ggattgaggt ctggggtgac cggcaccggc cgataattga
      361 tatcgggaat gatccgtacc gggttgatgg tatagagccg acgggcctgc gccggcacgg
      421 ttgaggcagg atagtgcagg cctagttttg actcgacctc ggcgcaccga tcctctgcga
      481 tcacttcgcc gctgaagtcg gaggcgaagc gatagatcat cacccgatcg aagccggtaa
      541 tcttccgcac ctcttgcgcc gcggcggcgc aggcgctttc caaggtttcg gcggcctgca
      601 ggcggcggat ggcgctgttg gtgcggcgga agaacgcctg cggctcggcg acgtcccgct
      661 ggggaggctc gagctcgagg aagatgagct gatcatggcg atgccaggag ccgatgaagc
      721 ctgcgtcctt tcgcatcgtg aagccgacag tgatcggtgc tccgacggcc gccccgggct
      781 cggccaaggc gatcgtcaga cggttgtgcg tctccgagtc gaagacatcg gccgcagagc
      841 ggccgatcag ggcgccgatc gccagtccgg tgagttcggg aaggttgtcg ctgccggcaa
      901 cgatcgtcat gtcggcggcg agggcgagca gcagtccatg cggttggatg gcaccgggga
      961 tatggatcgg ctcatcgtcg caggtcaaga ggtcaggctg cctggcgacg gatccttcag
     1021 ccatggagag atctgcgcaa aagtcct
//
