LOCUS       sequence-71078-e        1113 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An AAV vector that expresses iRFP under the c-fos promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Richie CT, Whitaker LR, Whitaker KW, Necarsulmer J, Baldwin HA, 
            Zhang Y, Fortuno L, Hinkle JJ, Koivula P, Henderson MJ, Sun W, Wang 
            K, Smith JC, Pickel J, Ji N, Hope BT, Harvey BK
  TITLE     Near-infrared fluorescent protein iRFP713 as a reporter protein for 
            optogenetic vectors, a transgenic Cre-reporter rat, and other 
            neuronal studies.
  JOURNAL   J Neurosci Methods. 2017 Jun 1;284:1-14. doi: 
            10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2.
  PUBMED    28380331
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci 
            Methods."; date: "2017-06-1"; volume: "284:1-14. doi"; pages: " 
            10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2"
COMMENT     Sequence Label: pOTTC475 - pAAV c-fos iRFP
FEATURES             Location/Qualifiers
     source          1..1113
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             121..1068
                     /codon_start=1
                     /product="phytochrome-based near-infrared fluorescent 
                     protein, originally termed iRFP (Filonov et al., 2011)"
                     /label=iRFP713
                     /note="name was changed to iRFP713 (Shcherbakova and 
                     Verkhusha, 2013)"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
ORIGIN
        1 ttctctctcc ctgcttcatg cgacactagg gccacttgct ccacctgtgt ctggaacctc
       61 ctcgctcacc tccgctttcc tctttttgtt ttgtttcagg acttttgcgc agatctctcc
      121 atggctgaag gatccgtcgc caggcagcct gacctcttga cctgcgacga tgagccgatc
      181 catatccccg gtgccatcca accgcatgga ctgctgctcg ccctcgccgc cgacatgacg
      241 atcgttgccg gcagcgacaa ccttcccgaa ctcaccggac tggcgatcgg cgccctgatc
      301 ggccgctctg cggccgatgt cttcgactcg gagacgcaca accgtctgac gatcgccttg
      361 gccgagcccg gggcggccgt cggagcaccg atcactgtcg gcttcacgat gcgaaaggac
      421 gcaggcttca tcggctcctg gcatcgccat gatcagctca tcttcctcga gctcgagcct
      481 ccccagcggg acgtcgccga gccgcaggcg ttcttccgcc gcaccaacag cgccatccgc
      541 cgcctgcagg ccgccgaaac cttggaaagc gcctgcgccg ccgcggcgca agaggtgcgg
      601 aagattaccg gcttcgatcg ggtgatgatc tatcgcttcg cctccgactt cagcggcgaa
      661 gtgatcgcag aggatcggtg cgccgaggtc gagtcaaaac taggcctgca ctatcctgcc
      721 tcaaccgtgc cggcgcaggc ccgtcggctc tataccatca acccggtacg gatcattccc
      781 gatatcaatt atcggccggt gccggtcacc ccagacctca atccggtcac cgggcggccg
      841 attgatctta gcttcgccat cctgcgcagc gtctcgcccg tccatctgga attcatgcgc
      901 aacataggca tgcacggcac gatgtcgatc tcgattttgc gcggcgagcg actgtgggga
      961 ttgatcgttt gccatcaccg aacgccgtac tacgtcgatc tcgatggccg ccaagcctgc
     1021 gagctagtcg cccaggttct ggcctggcag atcggcgtga tggaagagtt gtacaagtaa
     1081 gaattcgata tcaagcttat cgataatcaa cct
//
