LOCUS       sequence-71080-e         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA expression on PiggyBac vector with Neo-selectable marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC103-PBNeo-U6-sgRNA-Expression
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     110..129
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        206..281
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa
       61 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat
      121 gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg
      181 aaacaccggg tcttcgagaa gacctgtttt agagctagaa atagcaagtt aaaataaggc
      241 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttgtt ttagagctag
      301 aaatagcaag ttaaaataag gctagtccgt ttttagcgcg tgcgccaatt ctgcagacaa
      361 atggcacgcg cctggatccc cgggtaccga gttgggagct cacggggaca gccccccccc
      421 aaagccccca gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg
      481 gggctccgct ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag
      541 cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt
      601 gagcctgcag acacctgggg ggatacgggg aaaaagcttt aggctgaaag agagatttag
      661 aatgacagtc tagtgggagc tcacggggac agcccccccc caaagccccc agggatgtaa
      721 ttacgtccct cccccgctag ggggcagcag cgagccgccc ggggctccgc tccgg
//
