LOCUS       sequence-71089-e        1140 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1140)
  TITLE     Prescission-cleavable N-terminal fusion vectors for E. coli 
            expression
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His6 prescission LIC cloning vector (HPKS)
FEATURES             Location/Qualifiers
     source          1..1140
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     69..88
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        69..87
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    88..112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             127..149
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             168..185
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             213..236
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     857..875
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             complement(939..959)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc
       61 cgcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga
      121 aataattttg tttaacttta agaaggagat ataccatggg ttcttctcac catcaccatc
      181 accatggttc ttctaacaac ctcgggatcg agctggaagt gctgttccag ggtccgaata
      241 ttcggaatga tttgattgcc aaaaatagga tctctggcat gcgagaatct cacgcaggca
      301 gttctatgag gcagagcgac acctttaggc agaccagtag atccagagga gttcatgatc
      361 agtgcaattg tcttgtccct atcgaaggac tctggcacaa aatcgtattc attaaaaccg
      421 ggaggtagat gagatgtgac gaacgtgtac atcgactgaa atccctggta atccgtttta
      481 gaatccatga taataatttt ttggatgatt gggagctttt tttgcacgtt caaaattttt
      541 tgcaacccct ttttggaaac gaacaccacg gtaggctgcg aaatgcccat actgttgagc
      601 aattcacgtt cattataaat gtcgttcgcg ggcgcaactg caactccgat aaataacgcg
      661 cccaacaccg gcataaagaa ttgaagagag ttttcactgc atacgacgat tctgtgattt
      721 gtattcagcc catatcgttt catagcttct gccaaccgaa cggacatttc gaagtactca
      781 gcgtaagtga tgtccacctc gatatgtgca tctgtaaaag caattgttcc aggaaccagg
      841 gcgtatctct tcatagcctt atgcagttgc tctccagcgg ttccatcttc cagcggatag
      901 aatggcgccg ggcctttctt tatgtttttg gctgcattgg attggaagta caggttttcg
      961 cccttcttgg cctttatgag gatctctctg atttttcttg cgtcgagttt tccggtaaga
     1021 cctttcggta cttcgtccac aaacacaact cctccgcgca actttttcgc ggttgttact
     1081 tgactggcga cgtaatccac gatctctttt tccgtcatcg tctttccgtg ctccaaaaca
//
