LOCUS       sequence-71090-e         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses MepTrac in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   De Michele R, Ast C, Loque D, Ho CH, Andrade SL, Lanquar V, 
            Grossmann G, Gehne S, Kumke MU, Frommer WB
  TITLE     Fluorescent sensors reporting the activity of ammonium transceptors 
            in live cells.
  JOURNAL   eLife. 2013 Jul 2;2:e00800.
  PUBMED    23840931
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2013-07-2"; volume: "2"; pages: "e00800"
COMMENT     Sequence Label: pDR-meptrac
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..286)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNH
                     YLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(77..98)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgcccttgct caccatagat ccaccggtac cacccttgta cagctcgtcc atgccgagag
       61 tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt
      121 ctttgctcag cttggactgg acgctcaggt agtggttgtc gggcagcagc acggggccgt
      181 cgccgatggg ggtgttctgc tggtagtggt aggcgagctg cacgccgccg tcctcgatgt
      241 tgtggcggat cttgaagttc gccttgatgc cgttcttctg cttgtcggcc ttgatataga
      301 cgttagacaa acgtgtaaca gggtcattac gcttacccag tatcaaagcg taaactagac
      361 caccatgtcc agatgttaaa tggacacata aaccacctgc atagtccaag ctacccaatt
      421 tgaccaacca accctcggca ttccagaccc agcatgcaat aggacaataa acaatagtca
      481 tccataaaaa caagaacacc atcataggaa acaaccttgc cctctcgcag gcaccaccta
      541 gcattagggc accggtgact gcggcaaaca taccttggta aacggcaaac agtatatcgg
      601 gaagagaact gacactagat ggggctccta aaacgttacg aaacccaaag aattccaagg
      661 taccaataaa accgttacct ctagtgttgt gtgagaaagc taatgagtat ccccagaaaa
      721 accattggaa aatacacacg gcggaagcca tcatcgaggc ccaaagcaag gataaagcat
      781 gctttttcct ggataaacca gaatataata aaccgatacc tgggaccata atccacacac
      841 ctgctgaagc cacaccgatc catcccatgt tggccaagtc aaattgtgta ttcaaatctg
      901 ttgtcaacga gttaccaccc gttccttcgc ctgtaggcgt acctgtaaaa ttgtaa
//
