LOCUS       sequence-71091-e        1064 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1064)
  TITLE     Prescission-cleavable N-terminal fusion vectors for E. coli 
            expression
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His6 prescission LIC cloning vector (HPKS)
FEATURES             Location/Qualifiers
     source          1..1064
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(37..54)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             993..1013
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 agcttccttt cgggctttgt tagcagccgg atctcagtgg tggtggtggt ggtgctcgag
       61 ctgcgatttt aagtgttgtt ccattccatc acggttttgg aatgtttact acactcggat
      121 atttgatatg tggatttcga gtcgtcttaa tgtatagatt tgaagaagag ctgtttctga
      181 ggagccttca ggattacaag attcaaagtg cgctgctggt gccaacccta ttctccttct
      241 tcgccaaaag cactctgatt gacaaatacg atttatctaa tttacacgaa attgcttctg
      301 gtggcgctcc cctctctaag gaagtcgggg aagcggttgc caagaggttc catctgccag
      361 gtatcaggca aggatatggg ctcactgaga ctacatcagc tattctgatt acacccgagg
      421 gggatgataa accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg aaggttgtgg
      481 atctggatac cgggaaaacg ctgggcgtta atcaaagagg cgaactgtgt gtgagaggtc
      541 ctatgattat gtccggttat gtaaacaatc cggaagcgac caacgccttg attgacaagg
      601 atggatggct acattctgga gacatagctt actgggacga agacgaacac ttcttcatcg
      661 ttgaccgcct gaagtctctg attaagtaca aaggctatca ggtggctccc gctgaattgg
      721 aatccatctt gctccaacac cccaacatct tcgacgcagg tgtcgcaggt cttcccgacg
      781 atgacgccgg tgaacttccc gccgccgttg ttgttttgga gcacggaaag acgatgacgg
      841 aaaaagagat cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag ttgcgcggag
      901 gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac gcaagaaaaa
      961 tcagagagat cctcataaag gccaagaagg gcgaaaacct gtacttccaa tccaatgcag
     1021 ccaaaaacat aaagaaaggc ccggcgccat tctatccgct ggaa
//
