LOCUS       sequence-71092-e        1163 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1163)
  TITLE     Prescission-cleavable N-terminal fusion vectors for E. coli 
            expression
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His6 MBP prescission LIC cloning vector (H...
FEATURES             Location/Qualifiers
     source          1..1163
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     69..88
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        69..87
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    88..112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             127..149
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             168..185
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc
       61 cgcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga
      121 aataattttg tttaacttta agaaggagat ataccatggg ttcttctcac catcaccatc
      181 accatggttc ttctatgaaa atcgaagaag gtaaactggt aatctggatt aacggcgata
      241 aaggctataa cggtctcgct gaagtcggta agaaattcga gaaagatacc ggaattaaag
      301 tcaccgttga gcatccggat aaactggaag agaaattccc acaggttgcg gcaactggcg
      361 atggccctga cattatcttc tgggcacacg accgctttgg tggctacgct caatctggcc
      421 tgttggctga aatcaccccg gacaaagcgt tccaggacaa gctgtatccg tttacctggg
      481 atgccgtacg ttacaacggc aagctgattg cttacccgat cgctgttgaa gcgttatcgc
      541 tgatttataa caaagatctg ctgccgaacc cgccaaaaac ctgggaagag atcccggcgc
      601 tggataaaga actgaaagcg aaaggtaaga gcgcgctgat gttcaacctg caagaaccgt
      661 acttcacctg gccgctgatt gctgctgacg ggggttatgc gttcaagtat gaaaacggca
      721 agtacgacat taaagacgtg ggcgtggata acgctggcgc gaaagcgggt ctgaccttcc
      781 tggttgacct gattaaaaac aaacacatga atgcagacac cgattactcc atcgcagaag
      841 ctgcctttaa taaaggcgaa acagcgatga ccatcaacgg cccgtgggca tggtccaaca
      901 tcgacaccag caaagtgaat tatggtgtaa cggtactgcc gaccttcaag ggtcaaccat
      961 ccaaaccgtt cgttggcgtg ctgagcgcag gtattaacgc cgccagtccg aacaaagagc
     1021 tggcaaaaga gttcctcgaa aactatctgc tgactgatga aggtctggaa gcggttaata
     1081 aagacaaacc gctgggtgcc gtagcgctga agtcttacga ggaagagttg gcgaaagatc
     1141 cacgtattgc cgccactatg gaa
//
