LOCUS       sequence-71094-e        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  TITLE     Prescission-cleavable N-terminal fusion vectors for E. coli 
            expression
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His6 MBP prescission LIC cloning vector (H...
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..67
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     688..706
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             complement(770..790)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gaacaacaac aacaataaca ataacaacaa cctcgggatc gagctggaag tgctgttcca
       61 gggtccgaat attcggaatg atttgattgc caaaaatagg atctctggca tgcgagaatc
      121 tcacgcaggc agttctatga ggcagagcga cacctttagg cagaccagta gatccagagg
      181 agttcatgat cagtgcaatt gtcttgtccc tatcgaagga ctctggcaca aaatcgtatt
      241 cattaaaacc gggaggtaga tgagatgtga cgaacgtgta catcgactga aatccctggt
      301 aatccgtttt agaatccatg ataataattt tttggatgat tgggagcttt ttttgcacgt
      361 tcaaaatttt ttgcaacccc tttttggaaa cgaacaccac ggtaggctgc gaaatgccca
      421 tactgttgag caattcacgt tcattataaa tgtcgttcgc gggcgcaact gcaactccga
      481 taaataacgc gcccaacacc ggcataaaga attgaagaga gttttcactg catacgacga
      541 ttctgtgatt tgtattcagc ccatatcgtt tcatagcttc tgccaaccga acggacattt
      601 cgaagtactc agcgtaagtg atgtccacct cgatatgtgc atctgtaaaa gcaattgttc
      661 caggaaccag ggcgtatctc ttcatagcct tatgcagttg ctctccagcg gttccatctt
      721 ccagcggata gaatggcgcc gggcctttct ttatgttttt ggctgcattg gattggaagt
      781 acaggttttc gcccttcttg gcctttatga ggatctctct gatttttctt gcgtcgagtt
      841 ttccggtaag acctttcggt acttcgtcca caaacacaac tcctccgcgc aactttttcg
      901 cggttgttac ttgactggcg acgtaatcca cgatctcttt ttccgtcatc gtctttccgt
      961 gctccaaaac aacaacggcg gcgggaagtt caccggcgtc atcgtcggga agacctgcga
     1021 cac
//
