LOCUS       sequence-71095-e        1041 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1041)
  TITLE     Prescission-cleavable N-terminal fusion vectors for E. coli 
            expression
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His6 MBP prescission LIC cloning vector (H...
FEATURES             Location/Qualifiers
     source          1..1041
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..53)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             992..1012
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gcttcctttc gggctttgtt agcagccgga tctcagtggt ggtggtggtg gtgctcgagc
       61 tgcgatttta agtgttgttc cattccatca cggttttgga atgtttacta cactcggata
      121 tttgatatgt ggatttcgag tcgtcttaat gtatagattt gaagaagagc tgtttctgag
      181 gagccttcag gattacaaga ttcaaagtgc gctgctggtg ccaaccctat tctccttctt
      241 cgccaaaagc actctgattg acaaatacga tttatctaat ttacacgaaa ttgcttctgg
      301 tggcgctccc ctctctaagg aagtcgggga agcggttgcc aagaggttcc atctgccagg
      361 tatcaggcaa ggatatgggc tcactgagac tacatcagct attctgatta cacccgaggg
      421 ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga aggttgtgga
      481 tctggatacc gggaaaacgc tgggcgttaa tcaaagaggc gaactgtgtg tgagaggtcc
      541 tatgattatg tccggttatg taaacaatcc ggaagcgacc aacgccttga ttgacaagga
      601 tggatggcta cattctggag acatagctta ctgggacgaa gacgaacact tcttcatcgt
      661 tgaccgcctg aagtctctga ttaagtacaa aggctatcag gtggctcccg ctgaattgga
      721 atccatcttg ctccaacacc ccaacatctt cgacgcaggt gtcgcaggtc ttcccgacga
      781 tgacgccggt gaacttcccg ccgccgttgt tgttttggag cacggaaaga cgatgacgga
      841 aaaagagatc gtggattacg tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg
      901 agttgtgttt gtggacgaag taccgaaagg tcttaccgga aaactcgacg caagaaaaat
      961 cagagagatc ctcataaagg ccaagaaggg cgaaaacctg tacttccaat ccaatgcagc
     1021 caaaaacata aagaaaggcc c
//
