LOCUS       sequence-71097-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length MSP1 ectodomain
            with no N-linked glycans upon cotransfection with BirA in mammalian 
            cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: MSP1-bio
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     493..512
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      535..544
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aaccgtcaga tcctcactct cttccgcatc gctgtctgcg agggccagct gttgggctcg
       61 cggttgagga caaactcttc gcggtctttc cagtactctt ggatcggaaa cccgtcggcc
      121 tccgaacggt actccgccac cgagggacct gagcgagtcc gcatcgaccg gatcggaaaa
      181 cctctcgaga aaggcgtcta accagtcaca gtcgcaaggt aggctgagca ccgtggcggg
      241 cggcagcggg tggcggtcgg ggttgtttct ggcggaggtg ctgctgatga tgtaattaaa
      301 gtaggcggtc ttgagacggc ggatggtcga ggtgaggtgt ggcaggcttg agatccagct
      361 gttggggtga gtactccctc tcaaaagcgg gcattacttc tgcgctaaga ttgtcagttt
      421 ccaaaaacga ggaggatttg atattcacct ggcccgatct ggccatacac ttgagtgaca
      481 atgacatcca ctttgccttt ctctccacag gtgtccactc ccaggtccaa gtttgccacc
      541 atggagtttc agacccaggt actcatgtcc ctgctgctct gcatgtctgg tgcggccgcc
      601 gtgacccacg agagctacca ggaactggtc aagaagctgg aagccctgga agatgccgtg
      661 ctgaccggct acagcctgtt ccagaaagaa aagatggtgc tgaacgagga agagatcacc
      721 accaagggcg cctctgccca gagtggagcc tctgctcagt ctggggcttc tgcccagtct
      781 ggtgcctcag cacagagcgg cgcttcagca cagtcaggcg caagcgcaca gagcggaaca
      841 tctggcccta gcggcccttc tggcaccagc cccagcagca gaagcaacac cctgccccg
//
