LOCUS       sequence-71103-e        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S
  TITLE     A modular cloning system for standardized assembly of multigene 
            constructs.
  JOURNAL   PLoS One. 2011 Feb 18;6(2):e16765. doi: 
            10.1371/journal.pone.0016765.
  PUBMED    21364738
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011-02-18"; volume: "6(2):e16765. doi"; pages: " 
            10.1371/journal.pone.0016765"
COMMENT     Sequence Label: pICH82094
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     127..144
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    384..408
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 cctatggaaa aacggctttg ccgcggccct ctcacttccc tgttaagtat cttcctggca
       61 tcttccagga aatctccgcc ccgttcgtaa gccatttccg ctcgccgcag tcgaacgacc
      121 gagcgtagcg agtcagtgag cgaggaagcg gaatatatcc tgtatcacat attctgctga
      181 cgcaccggtg cagccttttt tctcctgcca catgaagcac ttcactgaca ccctcatcag
      241 tgccaacata gtaagccagt atacactccg ctagcgccct gctcggatct gttggaccgg
      301 acagtagtca tggttgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg
      361 tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt
      421 agacaactta ataacacatt gcggacgttt ttaatgtact ggggttgaac actctgtgaa
      481 gacaatgcct gagacctgtc agaccaagtt tactcatata tactttagat tgatttaaaa
      541 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
      601 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
      661 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
      721 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
      781 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
      841 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
      901 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      961 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     1021 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaa
//
