LOCUS       sequence-71108-e         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses AmTrac-100 in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   De Michele R, Ast C, Loque D, Ho CH, Andrade SL, Lanquar V, 
            Grossmann G, Gehne S, Kumke MU, Frommer WB
  TITLE     Fluorescent sensors reporting the activity of ammonium transceptors 
            in live cells.
  JOURNAL   eLife. 2013 Jul 2;2:e00800.
  PUBMED    23840931
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2013-07-2"; volume: "2"; pages: "e00800"
COMMENT     Sequence Label: pDR-AmTrac-100
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..286)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNH
                     YLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(77..98)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgcccttgct caccatagat ccaccggtac cacccttgta cagctcgtcc atgccgagag
       61 tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt
      121 ctttgctcag cttggactgg acgctcaggt agtggttgtc gggcagcagc acggggccgt
      181 cgccgatggg ggtgttctgc tggtagtggt aggcgagctg cacgccgccg tcctcgatgt
      241 tgtggcggat cttgaagttc gccttgatgc cgttcttctg cttgtcggcc ttgatataga
      301 cgttctcgag tttctcgaac cgaccacgac gaggaccttc aataagagca ccccataaac
      361 ctgctatgcc accaaccatg tgaacaacac cggagccagc aaagtcaatg gctccggtgc
      421 taaacaaacg atcatccgct gaacgaaagg gactggccca tccatccggg gaccaaaacc
      481 agtgagagac aaccgggtaa acaaatccgg ttaagaaaga agagtatatc aagtaagcca
      541 cgaactgagt cctctctgcg atcgaaccac ttgtgattcc agcggcctcg attgcgaacg
      601 cccattggta gaggaagaaa gagtaatcag ctgtgggagt cggaaagtct ctaagagcaa
      661 agttgtgtct tccaatgaac ccttcggagg atcctccaaa ggcaaaggcg taaccaaaga
      721 gatagtagaa gagtcctccg gctgcagcgt caaggacatt ggtaagcatg atgttcatcg
      781 tattcttggc tctaacagaa ccagcacaaa gcatag
//
