LOCUS       sequence-71113-e         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length Pf92 ectodomain
            with no N-linked glycans upon cotransfection with BirA in mammalian 
            cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: Pf92-bio
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     484..503
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      526..535
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atcctcactc tcttccgcat cgctgtctgc gagggccagc tgttgggctc gcggttgagg
       61 acaaactctt cgcggtcttt ccagtactct tggatcggaa acccgtcggc ctccgaacgg
      121 tactccgcca ccgagggacc tgagcgagtc cgcatcgacc ggatcggaaa acctctcgag
      181 aaaggcgtct aaccagtcac agtcgcaagg taggctgagc accgtggcgg gcggcagcgg
      241 gtggcggtcg gggttgtttc tggcggaggt gctgctgatg atgtaattaa agtaggcggt
      301 cttgagacgg cggatggtcg aggtgaggtg tggcaggctt gagatccagc tgttggggtg
      361 agtactccct ctcaaaagcg ggcattactt ctgcgctaag attgtcagtt tccaaaaacg
      421 aggaggattt gatattcacc tggcccgatc tggccataca cttgagtgac aatgacatcc
      481 actttgcctt tctctccaca ggtgtccact cccaggtcca agtttgccac catggagttt
      541 cagacccagg tactcatgtc cctgctgctc tgcatgtctg gtgcggccgc caacgtggcc
      601 ttcgagcaga aggtgcagac caacctggcc cacgacaaga acggcgacat cgtgtacggc
      661 caccgggagt tcaagggcgg catctacgcc tttctgggct acgacaactg cgccatcgaa
      721 gtgaacaagg ccgtgaacgg catcgactgg aacgagaaga aagacgtgaa ggtgtccggc
      781 aacaac
//
