LOCUS       sequence-71124-e        1101 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1101)
  TITLE     Series-9: N- or C-terminal Tag E.coli T7 Expression Vectors with 
            Biobrick Polycistronic Restriction Sites
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET MYFQSNA tagged cloning vector with BioBric...
FEATURES             Location/Qualifiers
     source          1..1101
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     69..88
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        69..87
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             164..186
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(323..341)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      337..384
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(750..755,756..772,773..778))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(805..823)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        891..995
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc
       61 cgcgaaatta atacgactca ctatagggag accacaacgg tttccctcta gtgccggctc
      121 cggagagctc tttaatgcgg ccgcttaatt aactagaaat aattttgttt aactttaaga
      181 aggagatata catatgtact tccaatccaa tattggaagt ggataacgga tcgcgatcgc
      241 cctgcaggcc ggccggtacc acgcgtgcgc gctgatccgg ctgctaacaa agcccgaaag
      301 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
      361 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac
      421 gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac
      481 tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat
      541 caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat
      601 atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt
      661 gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg
      721 ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac
      781 atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
      841 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
      901 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
      961 gataaatgct tcaataacat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     1021 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     1081 tgaaagtaaa agatgctgaa g
//
