LOCUS       sequence-71143-e         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length AMA1 ectodomain
            with no N-linked glycans upon cotransfection with BirA in mammalian 
            cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: AMA1-bio
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..27
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     508..527
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      550..559
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agagctcgtt tagtgaaccg tcagatcctc actctcttcc gcatcgctgt ctgcgagggc
       61 cagctgttgg gctcgcggtt gaggacaaac tcttcgcggt ctttccagta ctcttggatc
      121 ggaaacccgt cggcctccga acggtactcc gccaccgagg gacctgagcg agtccgcatc
      181 gaccggatcg gaaaacctct cgagaaaggc gtctaaccag tcacagtcgc aaggtaggct
      241 gagcaccgtg gcgggcggca gcgggtggcg gtcggggttg tttctggcgg aggtgctgct
      301 gatgatgtaa ttaaagtagg cggtcttgag acggcggatg gtcgaggtga ggtgtggcag
      361 gcttgagatc cagctgttgg ggtgagtact ccctctcaaa agcgggcatt acttctgcgc
      421 taagattgtc agtttccaaa aacgaggagg atttgatatt cacctggccc gatctggcca
      481 tacacttgag tgacaatgac atccactttg cctttctctc cacaggtgtc cactcccagg
      541 tccaagtttg ccaccatgga gtttcagacc caggtactca tgtccctgct gctctgcatg
      601 tctggtgcgg ccgcccanaa ttactgggag cacccttacc agaacagcga cgtgtaccgg
      661 cccatcaacg agcacagaga gcaccccaaa gagtacgagt accccctgca ccaggaacac
      721 acctaccagc aggaagatag cggcgaggac ganaacaccc tgcagcacgc ctaccccatc
      781 gatcacgagg gcgccgaacc agcccccca
//
