LOCUS       sequence-71144-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length EBA140 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: EBA140-bio
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     495..514
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      537..546
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tgaaccgtca gatcctcact ctcttccgca tcgctgtctg cgagggccag ctgttgggct
       61 cgcggttgag gacaaactct tcgcggtctt tccagtactc ttggatcgga aacccgtcgg
      121 cctccgaacg gtactccgcc accgagggac ctgagcgagt ccgcatcgac cggatcggaa
      181 aacctctcga gaaaggcgtc taaccagtca cagtcgcaag gtaggctgag caccgtggcg
      241 ggcggcagcg ggtggcggtc ggggttgttt ctggcggagg tgctgctgat gatgtaatta
      301 aagtaggcgg tcttgagacg gcggatggtc gaggtgaggt gtggcaggct tgagatccag
      361 ctgttggggt gagtactccc tctcaaaagc gggcattact tctgcgctaa gattgtcagt
      421 ttccaaaaac gaggaggatt tgatattcac ctggcccgat ctggccatac acttgagtga
      481 caatgacatc cactttgcct ttctctccac aggtgtccac tcccaggtcc aagtttgcca
      541 ccatggagtt tcagacccag gtactcatgt ccctgctgct ctgcatgtct ggtgcggccg
      601 ccatcatcgg ccggaccctg tacaaccggc aggacgagag cagcgacatc agcagagtga
      661 acagccccga gctgaacaac aaccacaaga ccaacatcta cgacagcgac tacgaggacg
      721 tgaacaacaa gctgatcaac agcttcgtgg agaacaaggc cgtgaagaag aagcggagcc
      781 tgagcttcat caacaacaag gccaagagct acgacatcat cccccccagc tacagctacc
      841 ggaacgacaa gttcaacagc ctgagcgaga acgaggacaa cagcggca
//
