LOCUS       sequence-71168-e         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S
  TITLE     A modular cloning system for standardized assembly of multigene 
            constructs.
  JOURNAL   PLoS One. 2011 Feb 18;6(2):e16765. doi: 
            10.1371/journal.pone.0016765.
  PUBMED    21364738
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011-02-18"; volume: "6(2):e16765. doi"; pages: " 
            10.1371/journal.pone.0016765"
COMMENT     Sequence Label: pICH86988
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    182..203
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(218..223,224..241,242..248)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    256..272
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     261..283
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     280..296
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     280..296
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             292..615
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    309..365
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(366..383)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(366..382)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(375..397)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(591..610)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gaagttcatt tcatttggag aggacacgct cgagtataag agctcatttt tacaacaatt
       61 accaacaaca acaaacaaca aacaacatta caattacatt tacaattatc gatacaatgg
      121 gagaccgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
      181 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
      241 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
      301 attacgccaa gcttgcatgc ctgcaggtcg actctagagg atccccgggt accgagctcg
      361 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
      421 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
      481 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt
      541 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
      601 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
      661 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgacggtctc agcttgtcct
      721 gctttaatga gatatgcgag aagcctatga tcgcatgata tttgctttca attctgttgt
      781 gcacgttgta aaaaacctga gcatgtgtag ctcagatcct taccgccggt ttcggttcat
      841 tctaatgaat atatcacccg ttactatcgt atttttatga ataatattct ccgttcaatt
      901 tactgattgt accctactac ttatatgtac aatattaaaa tgaaaacaat atattgtgct
      961 gaataggttt atagcgacat ctatgataga gcgcc
//
