LOCUS       sequence-71172-e         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length PTRAMP 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: PTRAMP-bio
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     494..513
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      536..545
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gaaccgtcag atcctcactc tcttccgcat cgctgtctgc gagggccagc tgttgggctc
       61 gcggttgagg acaaactctt cgcggtcttt ccagtactct tggatcggaa acccgtcggc
      121 ctccgaacgg tactccgcca ccgagggacc tgagcgagtc cgcatcgacc ggatcggaaa
      181 acctctcgag aaaggcgtct aaccagtcac agtcgcaagg taggctgagc accgtggcgg
      241 gcggcagcgg gtggcggtcg gggttgtttc tggcggaggt gctgctgatg atgtaattaa
      301 agtaggcggt cttgagacgg cggatggtcg aggtgaggtg tggcaggctt gagatccagc
      361 tgttggggtg agtactccct ctcaaaagcg ggcattactt ctgcgctaag attgtcagtt
      421 tccaaaaacg aggaggattt gatattcacc tggcccgatc tggccataca cttgagtgac
      481 aatgacatcc actttgcctt tctctccaca ggtgtccact cccaggtcca agtttgccac
      541 catggagttt cagacccagg tactcatgtc cctgctgctc tgcatgtctg gtgcggccgc
      601 caatgacctg tgcagctgca acctgaaagt gcggaacaac tgggagcact tcctccaggt
      661 gtaccagaac aacggcaact tcaagatgga aatcctggaa cccgagtgca ccatccagaa
      721 gaatctgccc agcgaggaag agaaagagga attcatcgac ttcgaccgga aagagctgct
      781 gctgcacgac ttcgtggtca tcaagaacag ccagttcacc gacgagaaaa ccctggaact
      841 gtacctgagc aaagaggaaa acaacaaggc ctacctgatg ctgaccttct acctgggcga
      901 cctgaagctg atgatcggcc acaacagccc ctacgagatc agcctgatcc tgaacgtggc
      961 caccgtgaa
//
