LOCUS       sequence-71174-e        1116 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S
  TITLE     A modular cloning system for standardized assembly of multigene 
            constructs.
  JOURNAL   PLoS One. 2011 Feb 18;6(2):e16765. doi: 
            10.1371/journal.pone.0016765.
  PUBMED    21364738
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011-02-18"; volume: "6(2):e16765. doi"; pages: " 
            10.1371/journal.pone.0016765"
COMMENT     Sequence Label: pICH41822
FEATURES             Location/Qualifiers
     source          1..1116
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(556..573)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(556..572)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(565..587)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(781..800)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     900..922
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(960..978)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tgaccatgat tacgccaagc ttgcatgcct gcaggtcgac tctagaggat cccctttggg
       61 cccaggaagt tcggcgttgc tgggtattgc ccccatgccc ccgcttattt catttggttc
      121 ggcatcacct tcagctctca ttgactcctg gatttcagct aggcgtttag ctttctttgc
      181 ggcctggtgt ttcacctccc tactgtgttt cctgtggagc ttgttgagga aagtttctgc
      241 agggtttgag tattgatttc tgtctcctgc aggttcttta acttcttgac cgacatccat
      301 ctcgggcgtt ttcctgattg cagctaaatc ttcctctgcg tttaccgcat aagctggcat
      361 tcttgtgaag caatcaagca agtccccatc tgagaacaaa ctttccattt cctctacatc
      421 cgacacttcc cttggtcgga aggctttcca cgcttgcaag gggtggaatc tgaagtccca
      481 agtttccacg aagacaatcc ctcggtcaca tgtgcatcct cgctcttgtc ttcaaagggt
      541 accgagctcg aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
      601 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
      661 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat
      721 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
      781 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
      841 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccatctc
      901 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gtcgaaaggg
      961 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     1021 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     1081 ttcaaatatg tatccgctca tgagacaata accctg
//
