LOCUS       sequence-71179-e        1114 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S
  TITLE     A modular cloning system for standardized assembly of multigene 
            constructs.
  JOURNAL   PLoS One. 2011 Feb 18;6(2):e16765. doi: 
            10.1371/journal.pone.0016765.
  PUBMED    21364738
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011-02-18"; volume: "6(2):e16765. doi"; pages: " 
            10.1371/journal.pone.0016765"
COMMENT     Sequence Label: pICH50881
FEATURES             Location/Qualifiers
     source          1..1114
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(562..579)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(562..578)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(571..593)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(787..806)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     906..928
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(966..984)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tgaccatgat tacgccaagc ttgcatgcct gcaggtcgac tctagaggat cccctttggg
       61 cccaggaagt tcggcgttgc tgggtattgc ccccatgccc ccgcttattt catttggttc
      121 ggcatcacct tcagctctca ttgactcctg gatttcagct aggcgtttag ctttctttgc
      181 ggcctggtgt ttcacctccc tactgtgttt cctgtggagc ttgttgagga aagtttctgc
      241 agggtttgag tattgatttc tgtctcctgc aggttcttta acttcttgac cgacatccat
      301 ctcgggcgtt ttcctgattg cagctaaatc ttcctctgcg tttaccgcat aagctggcat
      361 tcttgtgaag caatcaagca agtccccatc tgagaacaaa ctttccattt cctctacatc
      421 cgacacttcc cttggtcgga aggctttcca cgcttgcaag gggtggaatc tgaagtccca
      481 agtttccacg aagacaatcc ctcggtcaca tgtgcatcct cggtctcata gtttgtcttc
      541 aagggtaccg agctcgaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
      601 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
      661 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
      721 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
      781 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
      841 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
      901 catctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagtcg
      961 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     1021 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     1081 aatacattca aatatgtatc cgctcatgag acaa
//
