LOCUS       sequence-71185-e        1108 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S
  TITLE     A modular cloning system for standardized assembly of multigene 
            constructs.
  JOURNAL   PLoS One. 2011 Feb 18;6(2):e16765. doi: 
            10.1371/journal.pone.0016765.
  PUBMED    21364738
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011-02-18"; volume: "6(2):e16765. doi"; pages: " 
            10.1371/journal.pone.0016765"
COMMENT     Sequence Label: pICH50866
FEATURES             Location/Qualifiers
     source          1..1108
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(561..578)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(561..577)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(570..592)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(786..805)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     905..927
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(965..983)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tgaccatgat tacgccaagc ttgcatgcct gcaggtcgac tctagaggat ccccttgaag
       61 acaatgccga attcgatatc gcacatgtga ccgagggatt gtcttcgtgg aaacttggga
      121 cttcagattc caccccttgc aagcgtggaa agccttccga ccaagggaag tgtcggatgt
      181 agaggaaatg gaaagtttgt tctcagatgg ggacttgctt gattgcttca caagaatgcc
      241 agcttatgcg gtaaacgcag aggaagattt agctgcaatc aggaaaacgc ccgagatgga
      301 tgtcggtcaa gaagttaaag aacctgcagg agacagaaat caatactcaa accctgcaga
      361 aactttcctc aacaagctcc acaggaaaca cagtagggag gtgaaacacc aggccgcaaa
      421 gaaagctaaa cgcctagctg aaatccagga gtcaatgaga gctgaaggtg atgccgaacc
      481 aaatgaaata agcgggggca tgggggcaat acccagcaac gccgaacttc ctgggcccaa
      541 agggtaccga gctcgaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
      601 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
      661 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
      721 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
      781 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
      841 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
      901 atctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagtcga
      961 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     1021 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     1081 atacattcaa atatgtatcc gctcatga
//
