LOCUS       sequence-71193-e         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for co-expression of constitutively 
            active MKK4 and MKK7a1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Bose AK, Janes KA
  TITLE     A high-throughput assay for phosphoprotein-specific phosphatase 
            activity in cellular extracts.
  JOURNAL   Mol Cell Proteomics. 2013 Mar;12(3):797-806. doi: 
            10.1074/mcp.O112.024059. Epub 2012 Dec 11.
  PUBMED    23233447
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/mcp.O112.024059"; journalName: "Mol Cell Proteomics"; date:
            "2013-03"; volume: "12"; issue: "3"; pages: "797-806"
COMMENT     Sequence Label: pCDFDuet-1 MKK4(EE)-MKK7a1(EE)
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tgcggagtag tgattgccca tacattgttc agttttatgg tgcactcttc agagagggtg
       61 actgttggat ctgtatggaa ctcatgtcta cctcgtttga taagttttac aaatatgtat
      121 atagtgtatt agatgatgtt attccagaag aaattttagg caaaatcact ttagcaactg
      181 tgaaagcact aaaccactta aaagaaaact tgaaaattat tcacagagat atcaaacctt
      241 ccaatattct tctggacaga agtggaaata tcaagctctg tgacttcggc atcagtggac
      301 agcttgtaga cgagattgcc aaggaaagag atgctggctg taggccatac atggcacctg
      361 aaagaataga cccaagcgca tcacgacaag gatatgatgt ccgctctgat gtctggagtt
      421 tggggatcac attgtatgag ttggccacag gccgatttcc ttatccaaag tggaatagtg
      481 tatttgatca actaacacaa gtcgtgaaag gagatcctcc gcagctgagt aattctgagg
      541 aaagggaatt ctccccgagt ttcatcaact ttgtcaactt gtgccttacg aaggatgaat
      601 ccaaaaggcc aaagtataaa gagcttctga aacatccctt tattttgatg tatgaagaac
      661 gtgccgttga ggtcgcatgc tatgtttgta aaatcctgga tcaaatgcca gctactccca
      721 gctctcccat gtatgtcgat tgagtc
//
