LOCUS       sequence-71196-e        7116 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Provides the WUS promoter as GreenGate module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7116)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 7116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGA003
FEATURES             Location/Qualifiers
     source          1..7116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6702..6721)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6703..6721)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6731..6753)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6947..6966)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7066..7088
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tcccatgttt acgtttacgt tcacgttaat
     2281 gtcgtagata tacagacata atatcttgcc gaaaaataaa ttaaagaagt aaataaaaaa
     2341 aacctaacag aattatatat gcaaacaaca aagcatatag aaaagtaaat taagaaacat
     2401 tttaaaaaga atatagtatt gtattttaca taaatatttc tataaattaa tattcattaa
     2461 cttatacaat aaattaatat taatttatag gataaattaa tatcactata aattgataaa
     2521 atttcatggt cccaacatta ttaatttata aagtttctac tgtagtttta attcatgtgc
     2581 atagtagttt atagtttcta cttcttctta aagaatttca ataaaatagt tatataaatc
     2641 tcagatatca ttagtattta tatcctacta tattatttgg gaagtacatt ttaaatctaa
     2701 ccttaatttt tgtaattaat tacatgacaa tgccattaaa aaaaattaat caaaagaaaa
     2761 ttttttaata acgtcattaa ggttaccaaa atcatttaac gacaatattt atctcttaat
     2821 tatagggctt attggatcta aagacgggtc attaaagatt ttctaaactg aagcaaaata
     2881 taccgaatat tcaaatatct atctgttacc aaattataaa caaataagtt catatttaat
     2941 tttttatata cgaaatcaat taaatcttaa tcacataaaa attaacacaa aaaaaagtat
     3001 tcaacaatta aaaaattatc aaatctctgt gttatagaag tttttatact atattcgaat
     3061 aaagtgtaat ataccttttc aatttgtatt ctttaactaa tttaagctaa ctaatatatt
     3121 tgctaatctt atacctatca aaaccgacta tcttttctta tctttatagt atttttgcaa
     3181 gacttttagg tggatttggg ttaaagtgca ttcgggtcat atggtacatt ccttattaaa
     3241 taaatatggt gcactcctat gttattagct aaaatgttta gattataatt tagagtcata
     3301 tcataaataa aattaatatt aataaaataa atggcttttt ggcaagacgg atctggaggg
     3361 acgggttttt gaatcaacat taataaaaaa gtaaaatata attaatccac cgtttcaata
     3421 cgggttaaat ctttaatctt ttttttttta agaccactaa tattaaacat atcaaatcat
     3481 cctaatttag aaaagagtat ataaaactaa aaatgttatg tggtatgtat aatgttacta
     3541 tatataaaat taaagtataa aatataaatg tattagagaa tgataaaatt ttcaaaactt
     3601 atatatataa taaataattc ttaaatttta aaaattacta ctttaaaaaa aatcaaggga
     3661 cgggtaaaga aattacagaa cgggttttat tttggaattg ggttatatgg tagatgtatt
     3721 ttaatcaata tttagaatat ttataaaatt ttattaatat gttgttttga taagagttaa
     3781 aaacttcagt tttttaacaa ttgtctaatg tattcgtggt atagtgttac ttaataacaa
     3841 ttataaactg taaaatataa atattttata aaaataaaat tagcaagttt taatatatat
     3901 tacctttaaa aaataaatcg tcccgcgtta tatcgcgggt taaaatctag tcgatttata
     3961 tttacaagat cttaggttga aaataacata caaactacct tcgtctataa attaaatata
     4021 aattggcata atatctggaa aatttcttac aatcaagtca tgacgattga cgaatattga
     4081 aagctcaaac actaatgttg tacgttgatg aactaattaa aaattcaata aatgctctaa
     4141 acatataaac agtgtaacgt aaggtgtaaa tagcgaataa catatgacag actgaatata
     4201 aggctcggat cgtagagggg accatgttcc aaacccaatt ttcccccatt aatttccggg
     4261 aaaccaaaat aataaaagct aattataaac gttttcatga aattagggca aaagtacaaa
     4321 ctgaaactaa tgatgcccta aatgaaaatg aaatctctgc aataaagaag agtggcttta
     4381 ggggccacgt agacattagt tacaaaagct ctgcgtcttt tagccctctg cctcttcaac
     4441 agtactttac gacaatgaaa aagatcgtga tgcgtaaatt aagatcccct gtttctcact
     4501 gcatgctcgt taccgtacgt actcatagga gtttataaat caaagggcct atagagagta
     4561 ttctaagtag caaccactac gtaaagtttt accaaaaaaa aaaaccagta tgtaaatgtg
     4621 accatatgtt catgtagttg cttgtgtaca tctttttttt tttttttaac ttcaaacttt
     4681 atgctttatt atagtgaaga agtataatcc tcatggagtg tacatctata tatacactgc
     4741 atacgcatgt agagtaacaa gacattcttt gtttcttgtc aaaagacatt cacaatccaa
     4801 ctagagatat gattttatca tttgttagtt agtttatagt ttggtgactt ggtgtaatct
     4861 cctagttgga taaaaatatt ggaccgttga ttcgatagaa atgccaaacg acattacaaa
     4921 tcggatatat cgattggatg gaatccaaaa gtttatccaa tataatcgac taaagttaaa
     4981 aaatgagtaa tccaccagtc taactaaata ctttctttac caacaaaaat ttgggataca
     5041 actgagttta tttaggtgat aaaagattat aagacttaac cttttaacaa aaaaaatttt
     5101 ttaagacttc aatccttaga ttgttttcca aatattaata gttacaatag ttagtttgta
     5161 aaacctattt ttaattttcc gatactcttt gcacgttgtg ttgaatccga gaaatcatgg
     5221 gggctatata aacaaaacta tactatgaaa tagccaagca acttcagtga tcggatcttt
     5281 ggttttttat tttaaaaaaa ataaatattt tatcagccgt atcttcaggg ccattcccca
     5341 taaaaactat aatagcttct atgaaatttg tagattccta aaaaaatctt tatatatctt
     5401 ttatatacaa tatgtggctg ttacgtagaa caataagact atacaaaagt tgggtattgt
     5461 tccttttcaa ttacaaaagt catattcact aaaacaaagc ccttcaaact ttttaaaaaa
     5521 aatgaataaa tcacgcattt atacaaaaag atccatccat gctcctataa attcgagcat
     5581 ataattaact aaagaatact caacatgttc ataagtacac ctgtcttcac actcgtttca
     5641 cacattgtaa ttgacataaa ttttaatatc ttttaaagca aagcataagt aaaattatat
     5701 agagtatttc ttcaacacat ttccggacga tatcaccacg aaaaatatcc tatatcacct
     5761 atgtgtcatg actcatgacc ataaggaagg aaaaatgtat ttttatgatc aaacggtttt
     5821 cacttttttt attattcatt ctagaaaaaa attataatcg ttttctttca ttcctacgat
     5881 acaataattc ataacgatct tttagaattt ttgttttgtt ttctgtgtgt atgggtaaat
     5941 atctttcata cgtagacaga tacataacaa ttaaagctgg tgaagtaata attgtgaaga
     6001 gacagagaaa ttgaaaaaag agagtttgtt tttcgtctaa gtaaataccg atgatgccct
     6061 cacctttcta attccctcct ttgtcccttt ccattttcat gtttatttct aacttctctg
     6121 tccctttcaa tccttctcca ccagatatcc tctgattcga tcccccacaa attttttcat
     6181 aaaaattatt attattctca ctaaaactct caagagtagt ttcgattttt cctctgaata
     6241 aaaaaaaatc aaataatata tttgacaaga gaatttcctt cctaaacata acattttatt
     6301 tgaatgttaa ttcaattgat tatttttctt tcttatatcc ttttctttgg caaaatttat
     6361 ttcttatatc caagcacatt tttcaatagg gttttgtatg tatgaaatca tatctacatg
     6421 cataaaagaa taatatagtg aataactgat tttctagaat attgtaaaac cttctttttg
     6481 ggtccccatt tatatatata actcaatacc ttactatctc tctatatata tataatcttc
     6541 tctctcacac aaaacctaaa atctctttac taccagcaag ttgttttctt gctaacttca
     6601 aacttctctt tctcttgttc ctctctaagt cttgatctta tttaccgtta actttgtgaa
     6661 caaaagtcga atcaaacaca caacatgaga ccgaattctc gccctatagt gagtcgtatt
     6721 acctggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     6781 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     6841 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc
     6901 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
     6961 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     7021 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     7081 gtcagaggtt ttcaccgtca tcaccgaaac gcgcga
//
