LOCUS       sequence-71218-e        5151 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Provides alcR as GreenGate coding sequence module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 5151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGC011
FEATURES             Location/Qualifiers
     source          1..5151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4737..4756)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4738..4756)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4766..4788)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4982..5001)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5101..5123
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaggc tccatggcag atacgcgccg acgccagaat
     2281 catagctgcg atccctgtcg caagggcaag cgacgctgtg atgccccgga aaatagaaac
     2341 gaggccaatg aaaacggctg ggtttcgtgt tcaaattgca agcgttggaa caaggattgt
     2401 accttcaatt ggctctcatc ccaacgctcc aaggcaaaag gggctgcacc tagagcgaga
     2461 acaaagaaag ccaggaccgc aacaaccacc agtgaaccat caacttcagc tgcaacaatc
     2521 cctacaccgg aaagtgacaa tcacgatgcg cctccagtca taaactctca cgacgcgctc
     2581 ccgagctgga ctcaggggct actctcccac cccggcgacc ttttcgattt cagccactct
     2641 gctattcccg caaatgcaga agatgcggcc aacgtgcagt cagacgcacc ttttccgtgg
     2701 gatctagcca tccccggtga tttcagcatg ggccaacagc tcgagaaacc tctcagtccg
     2761 ctcagttttc aagcagtcct tcttccgccc catagcccga acacggatga cctcattcgc
     2821 gagctggaag agcagactac agatccggac tcggttaccg atactaatag tgtacaacag
     2881 gtcgctcaag atggatcgct atggtctgat cggcagtcgc cgctactgcc tgagaacagt
     2941 ctgtgcatgg cctcagacag cacagcacgg cgatatgccc gttccacaat gacgaagaat
     3001 ctgatgcgaa tctaccacga tagtatggag aatgcactgt cctgctggct gacagagcac
     3061 aattgtccat actccgacca gatcagctac ctgccgccca agcagcgggc ggaatggggc
     3121 ccgaactggt caaacaggat gtgcatccgg gtgtgccggc tagatcgcgt atctacctca
     3181 ttacgcgggc gcgccctgag tgcggaagag gacaaagccg cagcccgagc cctgcatctg
     3241 gcgatcgtag cttttgcgtc gcaatggacg cagcatgcgc agaggggggc tgggctaaat
     3301 gttcctgcag acatagccgc cgatgagagg tccatccgga ggaacgcctg gaatgaagca
     3361 cgccatgcct tgcagcacac gacagggatt ccatcattcc gggttatatt tgcgaatatc
     3421 atcttttctc tcacgcagag tgtgctggat gatgatgagc agcacggtat gggtgcacgt
     3481 ctagacaagc tactcgaaaa tgacggtgcg cccgtgttcc tggaaaccgc gaaccgtcag
     3541 ctttatacat tccgacataa gtttgcacga atgcaacgcc gcggtaaggc tttcaacagg
     3601 ctcccgggag gatctgtcgc atcgacattc gccggtattt tcgagacacc gacgccgtcg
     3661 tctgaaagcc cacagcttga cccggttgtg gccagtgagg agcatcgcag tacattaagc
     3721 cttatgttct ggctagggat catgttcgat acactaagcg ctgcaatgta ccagcgacca
     3781 ctcgtggtgt cagatgagga tagccagata tcatcggcat ctccaccaag gcgcggcgct
     3841 gaaacgccga tcaacctaga ctgctgggag cccccgagac aggtcccgag caatcaagaa
     3901 aagagcgacg tatggggcga cctcttcctc cgcacctcgg actctctccc agatcacgaa
     3961 tcccacacac aaatctctca gccagcggct cgatggccct gcacctacga acaggccgcc
     4021 gccgctctct cctctgcaac gcccgtcaaa gtcctcctct accgccgcgt cacgcagctc
     4081 caaaccctcc tctatcgcgg cgccagccct gcccgccttg aagcggccat ccagagaacg
     4141 ctctacgttt ataatcactg gacagcgaag taccaaccat ttatgcagga ctgcgttgct
     4201 aaccacgagc tcctcccttc gcgcatccag tcttggtacg tcattctaga cggtcactgg
     4261 catctagccg cgatgttgct agcggacgtt ttggagagca tcgaccgcga ttcgtactct
     4321 gatatcaacc acatcgacct tgtaacaaag ctaaggctcg ataatgcact agcagttagt
     4381 gcccttgcgc gctcttcact ccgaggccag gagctggacc cgggcaaagc atctccgatg
     4441 tatcgccatt tccatgattc tctgaccgag gtggcattcc tggtagaacc gtggaccgtc
     4501 gttcttattc actcgtttgc caaagctgcg tatatcttgc tggactgttt agatctggac
     4561 ggccaaggaa atgcactagc ggggtacctg cagctgcggc aaaattgcaa ctactgcatt
     4621 cgggcgctgc aatttctggg caggaagtcg gatatggcgg cgctggttgc gaaggattta
     4681 gagagaggtt tgaatgggaa agttgacagc tttttgtcag tgagaccgaa ttctcgccct
     4741 atagtgagtc gtattacctg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     4801 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     4861 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga
     4921 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
     4981 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     5041 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     5101 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg a
//
