LOCUS       sequence-71221-e         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides GFP:NLS as GreenGate coding sequence module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGC012
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(3..5,6..8,9..719)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(48..69)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(309..328)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     656..677
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
       61 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
      121 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
      181 ccctcgtgac caccttcacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
      241 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
      301 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
      361 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
      421 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
      481 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
      541 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
      601 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
      661 tcctgctgga gttcgtgacc gccgccggga tcactcacgg catggacgag ctgtacaaga
      721 ttgctgcagc ggccgaattc aagcgtgaag agcaagcaag gaaagctaag gtgaacaatg
      781 agaaaaagac ggaaatagtg aaaccagaga gttgtagcaa tgaaggagat gtcaaggatc
      841 tgaaaagaaa ggactctgag gatggaaacg agggtgagga agaagaagct tcttcgaaac
      901 cgaaaaagcc aaaagttgct ctttctcatc ttcaggacat tgacgacaca gaagctgatc
      961 aagaagaaga g
//
