LOCUS       sequence-71229-e        2267 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides 3xGFP as GreenGate coding sequence module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGC025
FEATURES             Location/Qualifiers
     source          1..2267
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1551..1553,1554..1556,1557..2267)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(1596..1617)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1857..1876)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2204..2225
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccatggtttc taaaggtgaa gagttgttta ctggagttgt gcctattctc gttgaacttg
       61 atggagatgt gaacggacac aagttctctg tttcaggaga aggagaggga gatgctactt
      121 atggaaaact tactttgaag tttatttgta ctactggaaa acttcctgtt ccttggccta
      181 ctttggtgac tactcttact tatggagtgc aatgcttctc aagataccca gatcacatga
      241 agcagcacga tttcttcaaa tctgctatgc ctgaaggtta tgttcaagag agaacaattt
      301 tctttaagga tgatggaaac tacaaaacta gagctgaggt taagtttgag ggagatacat
      361 tggtgaatag aattgaactc aagggaatcg atttcaaaga ggatggtaac atccttggac
      421 ataaattgga atacaactac aactcacaca acgtttacat catggctgat aagcaaaaga
      481 acggaattaa ggtgaacttc aagatcagac ataacatcga ggatggatct gttcagttgg
      541 ctgatcacta tcaacagaat actccaattg gagatggacc agttcttttg cctgataacc
      601 attacctttc tacacagtca aagctgtcta aagatcctaa tgagaaaaga gatcacatgg
      661 ttctccttga gtttgtgact gctgcaggta tcacactcgg tatggatgag ctttacaagg
      721 gtacctctgg atctggatca ggttctggta gtggttctgg ttcaggatct gcatgcatgg
      781 tttctaaggg tgaggaactc ttcactggtg ttgttcctat cctcgttgag cttgatggtg
      841 atgtgaacgg acataagttc tctgtttctg gtgaaggtga gggtgatgct acttacggaa
      901 agcttactct caagttcatc tgtaccactg gaaagttgcc tgttccttgg cctactctcg
      961 ttactacttt cacttacggt gtgcagtgct tctcaagata ccctgatcat atgaagcagc
     1021 acgatttctt caagtctgct atgcctgagg gatacgtgca agagagaact atcttcttca
     1081 aggatgatgg aaactacaag accagggctg aggttaagtt cgagggtgat actcttgtga
     1141 acaggatcga gcttaaggga atcgatttca aagaggatgg taacatcctt ggacacaagc
     1201 ttgagtacaa ctacaactct cacaacgtgt acatcatggc tgataagcag aagaacggaa
     1261 tcaaggttaa cttcaagatc aggcacaaca tcgaggatgg atctgttcag cttgctgatc
     1321 attaccagca gaacactcct attggagatg gacctgttct tctccctgat aaccattacc
     1381 tttctaccca gtctaagctc tctaaagatc ctaacgagaa gagggatcat atggtgctct
     1441 tggagtttgt tactgctgct ggaatcactc acggaatgga tgaactttac aagccgcggt
     1501 ctggaagtgg tagtggaagt ggatcaggta gtggatctgg aagtaccggt atggtgagca
     1561 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     1621 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     1681 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     1741 ccttcaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     1801 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     1861 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     1921 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     1981 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     2041 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     2101 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     2161 cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     2221 tcgtgaccgc cgccgggatc actcacggca tggacgagct gtacaag
//
